UDP-葡萄糖焦磷酸化酶galU基因对鼠伤寒沙门氏菌致病性的影响  

Effect of UDP-glucose Pyrophosphorylase galU Gene on the Pathogenicity of Salmonella typhimurium

作　　者：马帅 刘瑞雪 王玲[3] 江强世 宫晓 徐保娟 李彦 段笑笑 陈佳文 厉博雅 吕金东 宫鸿轩 杨胜男 雪芝蕊 郭莉莉 赵永达 Ma Shuai;Liu Ruixue;Wang Ling;Jiang Qiangshi;Gong Xiao;Xu Baojuan;Li Yan;Duan Xiaoxiao;Chen Jiawen;Li Boya;LüJindong;Gong Hongxuan;Yang Shengnan;Xue Zhirui;Guo Lili;Zhao Yongda(Qingdao Center for Animal Disease Prevention and Control,Qingdao 266033,Shandong,China;Jimo District Animal Husbandry Development Service Center,Qingdao 266228,Shandong,China;Qingdao Agricultural University,Qingdao 266109,Shandong,China;Qingdao Yibio Bioengineering Co.,Ltd.,Qingdao 266114,Shandong,China;Qingdao Bolin Biotechnology Co.,Ltd.,Qingdao 266114,Shandong,China)

机构地区：[1]青岛市动物疫病预防控制中心,山东青岛266033 [2]青岛市即墨区畜牧业发展服务中心,山东青岛266228 [3]青岛农业大学,山东青岛266109 [4]青岛易邦生物工程有限公司,山东青岛266114 [5]青岛博霖生物科技有限公司,山东青岛266114

出　　处：《中国动物检疫》2024年第12期110-117,共8页China Animal Health Inspection

基　　金：山东省泰山产业领军人才项目(TS20220701);中央引导地方资金项目(YDZX2023123);青岛市产业培育计划科技惠民专项(23-2-8-xdny-14-nsh,24-1-8-xdny-4-nsh)。

摘　　要：鼠伤寒沙门氏菌是一种重要的人兽共患病病原菌,近年来给我国养殖业造成了重大经济损失。galU基因编码UDP-葡萄糖焦磷酸化酶,在细菌生物膜合成过程中发挥着重要作用。为探究galU基因与鼠伤寒沙门氏菌致病性的相关性,从而为其致病机制研究奠定基础,利用Red/ET同源重组技术,将鼠伤寒沙门氏菌S584株galU基因敲除,并对基因缺失菌株进行了生物学特性及致病性等相关研究。结果显示:成功构建了galU基因敲除菌株S584AgalU,其与亲本菌株在适应期、对数期及稳定期的生长状态基本一致;缺失菌株丧失了生物被膜形成能力,且对DF-1细胞的粘附能力降低76.51%,对BALB/小鼠的致病性极显著降低(P<0.01)。结果表明,galU基因是鼠伤寒沙门氏菌重要的毒力因子。本研究为进一步阐明鼠伤寒沙门氏菌的致病机制和指导该病临床诊疗提供了理论依据。Salmonella typhimurium(S.typhimurium)is an important zoonotic pathogen,resulting in major economic loss to animal industry in China in recent years.UDP-glucose pyrophosphorylase is encoded by galU gene and plays an important role in the synthesis of bacterial bioflm.In order to explore the correlation of galU gene to the pathogenicity of S.typhimurium,and finally to support researches on the pathogenic mechanism of the bacterial strain,the galU gene of S.typhimurium S584 strain was knocked out using Red/ET homologous recombination technology,and the biological characteristics and pathogenicity of the gene-deleted mutant were studied.The results showed that a galUdeleted mutant,S584galU,was successfully constructed,and was generally consistent with its parental strain in the growth status at the stages of adaptation,logarithm and stability;the gene-deleted mutant lost its capacity of bioflm formation,reduced its adhesion to DF-1 cell by 76.51%,and its pathogenicity to BALB/c mice extremely significantly decreased(P<0.01).In conclusion,galU gene was an important virulence factor to S.typhimurium.A theoretical basis was provided by the study for further clarifying pathogenic mechanism of S.typhimurium and guiding clinical diagnosis and treatment for the disease.

关 键 词：鼠伤寒沙门氏菌 galU基因 生物学特性 致病性 

分 类 号：S851[农业科学—预防兽医学]