MIP-1α联合IL-8对小鼠CD34^+细胞体外增殖的影响  

The effect of MIP-1α combined IL-8 on mouse CD34^+ cells′ proliferation

作　　者：潘静[1] 李招权[1] 蒲晓允[1] 邓均[1] 黄辉[1] 

机构地区：[1]第三军医大学检验系临床血液学教研室,重庆400038

出　　处：《重庆医学》2002年第12期1182-1184,共3页Chongqing medicine

摘　　要：目的　了解巨噬细胞炎症蛋白 1α(macrophageinflammatoryprotein 1α ,MIP 1α)联合白细胞介素 8(interleukin 8IL 8)体外对小鼠CD34+ 细胞增殖的影响。方法　采用免疫磁珠吸附法分离小鼠CD34+ 细胞 ,将标本分为MIP 1α和IL 8各 1ng/ml组 ,10ng/ml组 ,5 0ng/ml组和空白组 ,建立液体培养体系及半固体培养体系 ,体系中分别加入相应浓度的MIP 1α和IL 8(空白组不加 ) ,培养一定时间后 ,观察各组的细胞生长情况 ,并作液体培养体系中细胞的周期分布检测。结果　 1ng/ml浓度的MIP 1α与相同浓度的IL 8联合使用 ,对小鼠CD34+ 细胞的体外增殖没有明显的影响 ,与空白组比较P >0 .0 5 ;10ng/ml和 5 0ng/ml浓度下 ,两者联合使用 ,则能明显抑制小鼠CD34+ 细胞的体外增殖 ,与空白组比较P <0 .0 1。但这两个浓度组相互比较却无显著差异 ,P >0 .0 5。周期分析结果表明 ,10ng/ml组和 5 0ng/ml组的G0 /G1期细胞均明显多于空白组和 1ng/ml组 ,P <0 .0 1,10ng/ml组与 5 0ng/ml组、1ng/ml组与空白组之间无显著差异 ,P >0 .0 5。结论　较低浓度下的MIP 1α联合IL 8,在体外能明显抑制小鼠CD34+ 细胞的增殖。Objective To study the effect of MIP 1αcombined IL 8 in different concentration on mouse CD34 + cell proliferation. Methods Mouse CD34 + cells were gotten from mouse femur bone marrow cells by MiniMACS, and then these CD34 + cells were cultivated in liquid system and 0.9% methyl cellulose system with different concentration of MIP 1αand IL 8 in different group. In order to know how about the proliferation of these CD34 + cells, the alive cell number, the clone formation units of CFU GM, CFU E, CFU Mix and the cell cycle were measured. Results (1) The alive cell number of 10ng/ml and 50ng/ml group of liquid system was significantly less than that of 1ng/ml and blank group ( P <0.01), but there was no difference between 10ng/ml and 50ng/ml group, so do that between 1ng/ml and blank group. The clone formation units experiment showed the same result as liquid system culture. (2) The G 0/G 1 cells of 10ng/ml and 50ng/ml group were significantly more than that of 1ng/ml and blank group, although there was no any difference between 10ng/ml and 50ng/ml group, also between 1ng/ml and blank group. Conclusion MIP 1α combined IL 8 in certain concentration can inhibit the proliferation of mouse CD34 + cells.

关 键 词：MIP-1Α IL-8 CD34^+细胞 细胞增殖 白细胞介素-8 免疫磁珠吸附法 巨噬细胞炎症蛋白-1Α 

分 类 号：Q253[生物学—细胞生物学]