彩色棉纤维色素提取和测定方法的研究  

Study on extraction and quantification of fiber pigment matter in colored cotton.

作　　者：王学德[1] 李悦有[1] 

机构地区：[1]浙江大学农学系,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2002年第6期596-600,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家高技术研究发展计划("863"计划;2001AA212081);转基因植物研究与产业化开发专项(J00-B-002-10);国家自然科学基金(30170058);国家教育部科研基金(重大0114)资助项目

摘　　要：以白色棉、棕色棉和绿色棉的成熟纤维为材料,用有机溶剂(乙醇、乙醚、二甲苯),酸性、碱性溶液,以及HNO3/乙醇溶液,分别对不同颜色纤维中的色素进行提取.结果表明,采用HNO3/乙醇溶液消煮法可以将彩色纤维中的色素物质提取出来.HNO3/乙醇的色素提取液在光谱波长为412nm处有一个最大吸收峰;颜色深浅不同的棕色或绿色棉纤维提取液在这一波长处的吸收值差异显著,随着纤维颜色的加深提取液吸收值增大(0.0634～0.9900);白色纤维的提取液在412nm处的吸收值很小(0.0072),可忽略不计.因此,用HNO3/乙醇法提取棉纤维色素和用分光光度计比色法可以定量比较彩色棉纤维的色泽深浅.在用酸性和碱性处理棕色和绿色棉纤维时,发现纤维颜色的深浅程度随处理溶液酸碱度的变化而变化,而且这种变化是可逆的.纤维中色素含量与纤维素含量存在负相关.Pigment matter in mature fibers of brown or green cotton was extracted by organic solvent (ethanol, aether and dimethylbenzene), acid and alkaline solution, and HNO 3/ethanol. The comparison of three extraction methods showed that pigment matter in colored fiber cells could be effectively extracted out only by HNO 3/ ethanol distillation. There was a maximum absorbance peak at 412 nm for fiber pigment solutions from HNO 3/ ethanol distillation. Absorbance readings of sample solutions at this wavelength were associated with fiber color and its intensity, for brown or green fibers increased with fiber color deepening (0.063 4 0.990 0) and for white fibers only had a negligible quantity (0.007 2). Therefor, it was suggested that the HNO 3/ethanol distillation method for extraction and quantification of fiber pigment be in favor of comparison of the color intensity for colored cotton varieties. When brown or green fibers were treated with acid or alkaline solutions, fiber color not only changed with pH value and but also restored to original color at pH7. There was a negative relationship between pigment and cellulose content in colored fibers.

关 键 词：彩色棉 纤维色素 提取技术 测定方法 

分 类 号：TS102.211[轻工技术与工程—纺织工程]