阴沟肠杆菌外膜蛋白缺失与头孢西丁耐药的关系  

Study of the relation of the lose 18 kda out membrane protein with resistance to cefoxitin in Enterobacter cloaceae.

作　　者：李向阳[1] 杨锦红[1] 陶洪群[1] 缪汉强[1] 

机构地区：[1]温州医学院附属第二医院检验科,325027

出　　处：《中华检验医学杂志》2002年第6期339-341,共3页Chinese Journal of Laboratory Medicine

摘　　要：目的　了解阴沟肠杆菌外膜上的外膜蛋白与头孢西丁耐药的关系。方法　参照美国临床实验室标准化委员会M1 0 0 S9文件的确认试验测定超广谱 β内酰胺酶 (ESBLs) ;超声破碎法提取ESBLs和外膜蛋白 (OMP) ,紫外分光光度法测定ESBLs活性 ,聚丙烯酰胺凝胶电泳 (SDS PAGE)分析OMP的成分。结果　头孢西丁耐药的 9株阴沟肠杆菌菌株中 ,有 6株菌株缺少分子量为 1 80 0 0蛋白区带 ,不产生头孢西丁水解酶 ,其中有 5株产生ESBLs;2株菌株有 1 80 0 0蛋白区带 ,产生头孢西丁水解酶 ;1株菌株不缺少外膜蛋白 ,产生ESBLs,但不产生头孢西丁水解酶。对头孢西丁敏感的产ESBLs菌株 ,不产生头孢西丁水解酶 ,也不缺少外膜蛋白。结论　 1 80 0Objective To study the relation of the porin at the out membrane with resistance to cefoxitin in Enterobacter cloaceae. Methods The extended spectrum beta lactamase(ESBLs) was detected by phenotypic confirmatory test according to NCCLS M100 S9;the beta lactamase and out membrane protein(OMP) was extracted by ultrasonication,the beta lactamase was detected by ultraviolet spectrophotometer,the composition of OMP was analyze by SDS PAGE. Results The 6 strains in 9 strains of Enterobacter cloaceae with resistance to cefoxitin lost 18 kda protein band and did not product hydrolase to cefoxitin and 5 strains was ESBLs positive. 2 strains producing hydrolase to cefoxitin did not lose the 18 kda protein. One strain with resistance to cefoxitin was producing ESBLs but did not defect the 18kda protein band and product hydrolase to cefoxitin. Conclusion The defect of the 18 kda protein band is nearly relation with resistance to cefoxitine in Enterobacter cloaceae.

关 键 词：阴沟肠杆菌 细菌外膜蛋白质 耐药性 Β内酰胺酶 头孢西丁 

分 类 号：R446.5[医药卫生—诊断学]