两种卵泡抑素免疫检测方法的比较  

Comparison of ELISA and IRMA for follistatin in biological materials

作　　者：董辉[1] 韩宏伟[1] 于春雷[1] 李一[1] 柳忠辉[1] 汪丽[2] 

机构地区：[1]吉林大学基础医学院免疫学系 [2]吉林大学中日联谊医院

出　　处：《中华检验医学杂志》2002年第6期342-344,共3页Chinese Journal of Laboratory Medicine

基　　金：吉林省科学技术厅课题基金资助项目( 534 59 1980 580 )

摘　　要：目的　建立高敏感度、特异性卵泡抑素 (FS)双位点酶联免疫吸附试验 (ELISA)法 ,并探讨其与免疫放射测定 (IRMA)法检测组织样本FS的应用价值。方法　采用重组人FS作为免疫原 ,制备针对FS的特异性单克隆抗体和多克隆抗体 ,利用抗FS单克隆抗体和多克隆抗体建立FS双位点ELISA法。结果　双位点ELISA法的敏感度为 1 2 5pg/ml,人血清FS回收率 86 %～ 1 0 4 % ,与重组人激活素A、牛抑制素、人促性腺激素、促黄体激素等无交叉反应。与IRMA法相比 ,两者均具有较高的特异性 ,但IRMA检测敏感度略低 (50 0pg/ml)。采用双位点ELISA法与IRMA法检测各种生物样本中的FS ,其结果显示二者相关性良好 (r≈ 0 .90 ,P <0 .0 1 )。Objective To establish a two site enzyme linked immunosorbent assay (ELISA) for follistatin, and compare ELISA with IRMA for detecting follistatin levels in biological material. Methods The monoclonal and polyclonal antibodies to follistatin were prepared by immunizing with recombinant human follistatin, and a sensitive and specifical two site ELISA for native follistatin was developed by using the monoclonal and polyclonal antibodies against follistatin. Results The sensitivity of two site ELISA was about 125 pg/ml and the recovery rate of follistatin was about 86%～104% in human serum. The two site ELISA had no significant cross reaction with rhActvin A, Inhibin, FSH, LH. The ELISA appeared to have higher sensitivity (125 pg/ml) than that of the IRMA (500 pg/ml), and There was higher correlation between using ELISA and IRMA for detecting the levels of follistatin in various biological meterials ( r ≈0.90, P <0.01). Conclusion The established ELISA can be used to detect the levels of follistatin in biological samples.

关 键 词：酶联免疫吸附测定 免疫放射测定 卵泡抑素 

分 类 号：R446.6[医药卫生—诊断学]