临床分离洋葱伯克霍尔德菌鉴定方法的探讨  

A identification method for clinical burkholderia cepacia isolates

作　　者：张军民[1] 罗燕萍[1] 赵莉萍[1] 白丽彦 姚艺辉[2] 

机构地区：[1]解放军总医院微生物科,北京100853 [2]厦门市中山医院检验科

出　　处：《中华检验医学杂志》2002年第6期333-335,共3页Chinese Journal of Laboratory Medicine

摘　　要：目的　探讨建立简便、实用鉴定临床分离洋葱伯克霍尔德菌的方法。方法　对临床分离的 38株菌和 5株标准菌株用表型和洋葱伯克霍尔德菌群特异引物聚合酶链反应 (PCR)进行鉴定确认 ,再用由三糖铁琼脂、氧化酶试验、产黄色素、触酶试验、纸片扩散法抗生素谱组成的方法进行鉴定。结果　PCR扩增阳性菌在三糖铁琼脂上均不利用葡萄糖且氧化酶阳性、触酶延迟反应 ,抗生素谱中亚胺培南耐药、头孢呋辛、复方新诺明敏感或中度耐药。在PCR阴性组中仅 1株临床菌触酶延迟反应 ,鉴定为伯克霍尔德菌属细菌。结论　利用三糖铁琼脂、氧化酶试验、触酶试验。Objective To approach a simple identification method for clinical Burkholderia cepacia isolates.Methods Thirty eight clinical isolates and 5 referenc strains were identified by phenotypic and genotypic methods. A simple method presented here included TSI agar, oxidase test, pigmentation test, catalase test and antibiograms.Results All but one B. cepacia isolate identified by phenotypic and genotypic methods were identified correctly by our method. One non B. Cepacia isolate identified by the genotypic method was identified as Burkholderia spp. by phenotyic and our methods.Conculsion The method we presented here was simple, practical for identification of clinical B. cepacia isolates.

关 键 词：洋葱伯克霍尔德菌 鉴定 聚合酶链反应 三糖铁琼脂 氧化酶试验 触酶试验 抗生素耐药谱 

分 类 号：R446.5[医药卫生—诊断学]