DNA与苯胺红T的相互作用与荧光定量检测  

Interaction Between Safranine T and Yeast DNA and Fluorescence-quantitative Determination of DNA

作　　者：叶宝芬[1] 朱永林[1] 鞠熀先[1] 

机构地区：[1]南京大学化学系,配位化学国家重点实验室,南京210093

出　　处：《高等学校化学学报》2002年第12期2253-2255,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 9975 013;2 9835 110 ) ;江苏省社会发展基金 (批准号 :BS2 0 0 10 6 3) ;教育部博士学科点基金 ( 2 0 0 0 0 2840 3);中国科学院长春应用化学研究所电分析化学开放课题资助

摘　　要：The interaction between safranine T(ST) and yeast DNA was studied by means of UV absorption and fluorescence spectrometry. The wavelengths of fluorescence excitation and emission of ST were 520 nm and 570 nm, respectively. The maximum absorption of UV spectrum was at 520 nm. Two kinds of interaction between ST and DNA, intercalation and electrostatic interaction, were conformed. The binding site size in DNA base pairs was 14.5±1.5 and the apparent binding constant was (2.81±0.11)×10 4 mol -1·L. The fluorescence quenching constant of DNA to ST was (1.52±0.19)×10 4 mol -1·L. A novel fluorescence analysis for DNA determination was presented and the effects of experimental conditions on the fluorescence quenching were discussed.The interaction between safranine T(ST) and yeast DNA was studied by means of UV absorption and fluorescence spectrometry. The wavelengths of fluorescence excitation and emission of ST were 520 nm and 570 nm, respectively. The maximum absorption of UV spectrum was at 520 nm. Two kinds of interaction between ST and DNA, intercalation and electrostatic interaction, were conformed. The binding site size in DNA base pairs was 14.5±1.5 and the apparent binding constant was (2.81±0.11)×10 4 mol -1·L. The fluorescence quenching constant of DNA to ST was (1.52±0.19)×10 4 mol -1·L. A novel fluorescence analysis for DNA determination was presented and the effects of experimental conditions on the fluorescence quenching were discussed.

关 键 词：相互作用 定量检测 DNA 苯胺红T 荧光猝灭 荧光分析 

分 类 号：Q523[生物学—生物化学] Q503