蛋白A连续床亲和毛细管色谱柱的制备及性能考察  

Preparation and Characterization of Protein A Immobilized on Monolithic Capillary Column for Affinity Chromatography

作　　者：潘再法[1] 莫卫民[1] 吴仁安[2] 罗权舟[2] 叶明亮[2] 邹汉法[2] 

机构地区：[1]浙江工业大学化工学院,杭州310014 [2]中国科学院大连化学物理研究所,国家色谱研究分析中心,大连116011

出　　处：《高等学校化学学报》2002年第11期2070-2072,共3页Chemical Journal of Chinese Universities

基　　金：国家杰出青年基金 (批准号 :2 972 5 5 12 )资助

摘　　要：An assay technique for the determination of the human IgG in human plasma has been developed by utilizing protein A immobilized monolithic capillary column. The affinity chromatography experiment was performed on a capillary electrophoresis instrument by using its pressure system as the driving force. Reactive monolithic capillary columns have been prepared by in-situ copolymerization of the monomers in the presence of porogenic dilute, and protein A was immobilized on the monolithic rods directly or through a 11 carbon atom space arm with molded synthetic methods. The non-specific adsorption of two kinds of media has been studied and the results showed that the medium without space arm gives very low non-specific adsorption of BSA. The affinity column without space arm was used to determine the human immunoglobulin G(HIgG) in human serum. The correlative coefficient of the calibration curve reaches 0 998 7. The total time of rapid analysis was less than 0 7 min. The consumption of eluent buffer and sample is much low than by conventional HPLC.An assay technique for the determination of the human IgG in human plasma has been developed by utilizing protein A immobilized monolithic capillary column. The affinity chromatography experiment was performed on a capillary electrophoresis instrument by using its pressure system as the driving force. Reactive monolithic capillary columns have been prepared by in-situ copolymerization of the monomers in the presence of porogenic dilute, and protein A was immobilized on the monolithic rods directly or through a 11 carbon atom space arm with molded synthetic methods. The non-specific adsorption of two kinds of media has been studied and the results showed that the medium without space arm gives very low non-specific adsorption of BSA. The affinity column without space arm was used to determine the human immunoglobulin G(HIgG) in human serum. The correlative coefficient of the calibration curve reaches 0 998 7. The total time of rapid analysis was less than 0 7 min. The consumption of eluent buffer and sample is much low than by conventional HPLC.

关 键 词：制备 性能 亲和色谱法 连续床毛细管色谱柱 蛋白A 人免疫球蛋白G 分析 

分 类 号：Q51[生物学—生物化学] Q503