产超广谱β-内酰胺酶变形杆菌耐药机制和分子流行病学研究  

Mechanism of Antibiotic-resistance and the Molecular Epidemiology of ESBLs-producing Proteus

作　　者：贾宁[1] 林茂虎[2] 陈世平[1] 索继江[1] 邢玉斌[1] 江朝光[1] 

机构地区：[1]解放军总医院,北京100853 [2]第一军医大学南方医院,广东广州510515

出　　处：《中华医院感染学杂志》2002年第11期811-813,共3页Chinese Journal of Nosocomiology

基　　金：自然科学基金资助项目 39970 6 2 9

摘　　要：目的　对产超广谱 β-内酰胺酶 (ESBL s)变形杆菌的耐药机制和分子流行病学进行初步探讨。方法　收集、鉴定菌株 ,进行 ESBL s检测、PCR扩增 bla TEM和 bla SHV基因、等电聚焦测定 β-内酰胺酶等电点和染色体DNA的 PFGE分析。结果　分离的 45株变形杆菌中有 7株产 ESBL s,bla TEM和 bla SHV基因扩增和等电聚焦结果显示 7株产 ESBL s变形杆菌全部为 TEM型 β-内酰胺酶 ;PFGE结果时间上和空间上未见有相关菌株的存在或明显的集中趋势。结论　合理使用抗生素对预防耐药菌的产生和控制医院感染十分重要 ;对患有产 ESBL s变形杆菌感染的患者应加强消毒隔离措施 ,防止医院感染的暴发和流行。OBJECTIVE To try to determine the mechanism of antibiotic-resistance and molecular epidemiology of ESBLs-producing Proteus. METHODS Isolates which had been collected and identified were subjected to drug susceptibility test, ESBLs detection, TEM-and SHV-β-lactamase gene PCR, β-lactamase isoelectric focusing and chromosomal DNA analysis by PFGE. RESULTS Seven ESBLs positive isolates of 45 strains produced TEM-type β-lactamase determined by PCR and isoelectric focusing. No close relative isolates were found by PFGE. CONCLUSIONS It is important to use antimicrobials correctly for preventing drug-resistant producing Proteus and diminishing their prevalence. Disinfection and isolation measures should still be enhanced towards the patients infected by ESBLs-producing Proteus to prevent nosocomial infection outbreak and prevalence.

关 键 词：产超广谱β-内酰胺酶变形杆菌 耐药机制 变形杆菌 超广谱Β-内酰胺酶 医院感染 分子流行病学 抗生素 

分 类 号：R378[医药卫生—病原生物学] R978.1[医药卫生—基础医学]