利用低强度脉冲电磁场对家猪精子电穿孔的研究  

Study on the Effects of Electroporation of Domestic Pig Sperm Cell Induced by Weak Electric-Magnetic Pulse Field

作　　者：王小行[1] 彭勇[1] 王子淑[1] 王保义[2] 张弘[2] 刘长军[2] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]四川大学无线电系,成都610064

出　　处：《四川大学学报（自然科学版）》2002年第5期934-937,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金 (6 0 0 710 2 4)

摘　　要：用低强度脉冲电磁场对家猪精子细胞进行电击处理 ,发现台盼蓝染料能够进入细胞 ,而且通过扫描电镜可以观察到细胞膜表面有明显的孔洞 .The sperms of domestic pig were freated with weak electric-magneti pulse field. The authors observe that Try-Pan blue can enter the sperm cells and there are obvious holes in the cell surface. It confirms that weak electric-magnetic pulse field can cause electroporation of domestic pig sperms and make the little exogenous molecules enter the sperm cells.

关 键 词：家猪 低强度脉冲电磁场 精子 电穿孔 转基因动物 DNA 电击法 

分 类 号：Q78[生物学—分子生物学] Q274