成纤维细胞生长因子-2对损伤面运动神经元代谢的影响  

EXOGENOUS FGF-2 REGULATES THE METABOLISM OF RAT FACIAL MOTONEURONS AFTER INJURY

作　　者：秦兆冰[1] 董明敏[1] 

机构地区：[1]郑州大学第一附属医院耳鼻咽喉科,郑州450052

出　　处：《解剖学报》2002年第6期590-593,共4页Acta Anatomica Sinica

基　　金：河南省科技攻关计划项目 (0 0 1170 664 )

摘　　要：目的　研究外源性成纤维细胞生长因子 2 (FGF 2 )对损伤面运动神经元降钙基因相关肽 (CGRP)和FGF 2表达的影响。　方法　采用大鼠面神经损伤模型 ,在损伤面神经近侧断端加入FGF 2 ,同时以生理盐水做对照 ,观察面运动神经元CGRP和FGF 2免疫阳性细胞数和灰度值。　结果　单纯损伤组和生理盐水组 ,损伤侧面运动神经元CGRP阳性细胞数和染色强度比对照侧明显增强 ,而FGF 2则表达下降 ;与生理盐水组比较 ,FGF 2处理组损伤侧面运动神经元CGRP阳性细胞数和染色强度明显下降 ,FGF 2阳性细胞数和染色强度增加。　结论　外源性FGF 2能增强面运动神经元FGF 2的表达 ,但对损伤面运动神经元CGRP的表达有负向调节作用。Objective To study the regulatory effect of exogenous fibroblast growth factor(FGF\|2) in expressions of calcitonin gene\|related peptide(CGRP) and FGF\|2 in facial motoneurons after injury. Methods The right facial nerve was transected 6?mm distal to the stylomastoid foraman.A 3?mm\+3 piece of Gelfoam presoaked in 25?μl of various test solutions(normal saline,or 16×10 6?IU/L FGF\|2,or 32×10 6?IU/L FGF\|2) was implanted adjacent to the proximal nerve stump.After three days survival,the rats were killed and brainstems were removed.Serial 20?μm cryosections were cut through the whole brainstem.The CGRP and FGF\|2 immunoreactivity were studied by immunohistochemisty and image analysis. Results An increase in the CGRP and decrease in the FGF\|2 signals were presented in axotomized motoneurons with saline\|treated animals.In FGF\|2\|treated animals,the lesion\|induced up\|regulation of CGRP and down\|regulation of FGF\|2 were attenuated compairing with normal saline treated animals.Conclusion\ Exogenous FGF\|2 can increase FGF\|2 expression and decrease CGRP expression in rat facial motoneurons after injury.\;[

关 键 词：面运动神经元 轴突损伤 降钙基因相关肽 成纤维细胞生长因子-2 大鼠 

分 类 号：R651.3[医药卫生—外科学]