脊髓慢性受压后神经性一氧化氮合酶表达的变化  

THE EXPRESSION OF nNOS IN THE CHRONIC SPINAL CORD COMPRESSIVE INJURIES

作　　者：夏玉军[1] 米曰堂[1] 迟焕方[1] 

机构地区：[1]青岛大学医学院解剖学教研室,青岛266021

出　　处：《解剖学报》2002年第6期599-602,共4页Acta Anatomica Sinica

基　　金：山东省卫生厅资助课题 [科鲁卫教字 (1999)第CA1号 ]

摘　　要：目的　探讨慢性脊髓压迫性损伤时 ,脊髓组织神经性一氧化氮合酶 (nNOS)免疫组织化学的变化及其与脊髓病理变化之间的关系。　方法　健康家兔 18只 ,随机分为正常对照组、实验对照组及实验组。实验组采用泛影葡胺囊逐级压迫复制慢性脊髓压迫动物模型 ,并持续逐级压迫脊髓 12周。取 3组家免相应脊髓节段 ,用Nissl染色观察脊髓的病理变化 ;用免疫组织化学方法对脊髓组织nNOS阳性运动神经元分布特点和nNOS含量变化进行分析。　结果　Nissl染色可见实验组脊髓压迫节段前角运动神经元损伤 ;实验组家免脊髓压迫节段前角nNOS阳性运动神经元较正常及实验对照组各个脊髓节段异常增加。　结论　在慢性脊髓压迫时 。Objective To study the immunohistochemistry change of neuronal nitric oxide synthase(nNOS) during the chronic spinal cord compression. Methods 18 healthy rabbits were randomly divided into normal,control and compressed groups.Membranous sac filled with cardiografin was applied to produce an animal model of chronic spinal cord compression.The sac was gradually enlarged resulting in chronic spinal cord compression in compressed groups for 12 weeks.Nissl's staining was applied to observe histopathological change and immunohistochemistry to nNOS change. Results The damage of motorneurons in the compressed block of compressed group was observed.No histopathological change was observed in normal and control groups.The number of nNOS positive motor neurons in the compressed block of compressed group was higher than that in the blocks of other groups.Conclusion\ The NO synthesis increased in chronic spinal cord injury.\;[

关 键 词：慢性脊髓压迫 神经性一氧化氮合酶 免疫组织化学 动物实验 形态学 

分 类 号：R651.2[医药卫生—外科学]