FGF受体在皮瓣移植中的分布变化  

THE DISTRIBUTION CHANGE OF THE FGF-R IN THE FLAP TRANSPLANTATIN

作　　者：刘芬兰[1] 赵建文[2] 闻杰[3] 刘远峰 柏树令[5] 李吉[5] 

机构地区：[1]沈阳医学院解剖学教研室 [2]沈阳医学院实验动物中心 [3]辽宁省东北电业中心医院外科 [4]沈铁北站医院放射科 [5]中国医科大学解剖学教研室,沈阳110034

出　　处：《解剖学报》2002年第6期642-646,共5页Acta Anatomica Sinica

基　　金：辽宁省自然科学基金资助项目 (9780 2 0 16);沈阳市科委资助项目 (964 13 5 0 42 9)

摘　　要：目的　研究皮瓣移植前后FGF受体分布特点及外源性bFGF对受体分布的影响。　方法　选择健康Wistar大鼠背部两侧随意皮瓣 (2cm× 1 5cm)模型 ,于原位缝合后即刻在皮瓣下注入bFGF ,应用SP免疫组织化学染色。　结果　大鼠皮瓣表皮基底层细胞 ,真皮层、皮下组织和深筋膜中的血管内皮细胞 ,成纤维细胞及毛囊、汗腺的上皮细胞都有FGF受体表达。FGF受体免疫反应阳性细胞数在皮瓣移植术后 1～ 5d逐渐增加 ,第 5d达高峰 ,第7d恢复到术前水平。加入外源性bFGF后与对照组相比实验组FGF受体免疫反应阳性细胞数在术后 1～ 3d都有明显增加 ,术后第 3d达高峰 ,高峰期比对照组提前 2d ,峰值也比对照组显著增高。　结论　大鼠皮瓣各层组织细胞中都存在FGF受体。FGF受体免疫反应阳性细胞数在术后 1～ 5d逐渐增加 ,第 5d达高峰。加入外源性bFGF后可使FGF受体增加。Objective To examine the chracteristics of distribution of the FGF R before and after the flap transplantation and the influence of exogenic bFGF on the distribution of FGF R. Method Caudally based random skin flaps were raised on the backs of Wistar rats,bFGF was instilled under flaps after closure.An immunohistochemical techniques stanining method was used. Results FGF receptors distributes in the stratum basal of epidermis,the vascular endothelial cells in the dermis and hypodermis,the fibroblasts and the epithelial cells of the hair follicle and the sweat gland.In compared with preoperation,the quantity of increased gradually from the 1st day to the fifth day after operation,and the peak value is on the fifth day,it then recovered to the pre operation level on the serventh day.After applying the exogenic bFGF,the positive immunoreactive cells in the experimental group,increased remarkably from the Ist day to the 3rd day after operation in contrast with the control group.The peak value is on the 3rd day which was two days earlier than that of the control group and the peak value of the experimental group is remarkably higher than that of the control group.Conclusion\ FGF R distributes in the stratum basal of epidermis,the vascular endothelial cells in the dermis and hypodermis,the fibroblasts,and the epithelial cells of the hair follicle and the sweat gland.In compaired with pre operation,the quantity of the positive immunoreactive cells of FGF R were increased gradually from the Ist day to fifth day after operation,and the peak value is on the fifth day.After applying the exogenic bFGF,the positive immunoreactive cells of FGF receptors were increased.\;[

关 键 词：皮瓣移植术 FGF受体 免疫组织化学 皮肤缺损 实验研究 

分 类 号：R622.1[医药卫生—整形外科]