+Gx负荷对大鼠脑组织能量代谢的影响  

Effects of +Gx Load on Energy Metabolism of Brain Tissue in Rats.

作　　者：吴斌[1] 谢宝生[1] 由广兴[1] 刘兴华[1] 路盛强[1] 黄伟芬[1] 

机构地区：[1]航天医学工程研究所,北京100094

出　　处：《航天医学与医学工程》2002年第6期406-409,共4页Space Medicine & Medical Engineering

基　　金：国家载人航天工程项目资助

摘　　要：目的观察 +Gx作用下大鼠脑组织能量代谢的变化特点 ,探讨其在 +Gx致脑功能改变和工作效率下降中的可能作用及防护措施。方法将 45只雄性Wistar大鼠随机分成对照组、+ 5Gx组、+ 1 0Gx组、+ 1 5Gx组和 + 2 0Gx组 ,分别暴露于相应G值的 +Gx条件下。之后 ,用高压液相色谱法测定脑组织中腺苷酸含量 ,用分光光度法测定乳酸 (LA)含量 ,用全自动生化分析仪测定乳酸脱氢酶 (LDH)活性。结果与对照组比 ,+ 5Gx、+ 1 0Gx、+ 1 5Gx、+ 2 0Gx作用 3min ,大鼠脑皮层LA含量明显增加 (P <0 .0 1 ) ;+1 0Gx、+ 1 5Gx、+ 2 0Gx作用 3min ,ADP含量和ADP/ATP、AMP/ATP比值明显增加 (P <0 .0 1 ) ,而ATP含量、EC以及LDH活性明显降低 (P <0 .0 5或 0 .0 1 ) ;+ 1 5Gx、+ 2 0Gx作用 3min ,AMP含量明显增加 (P <0 .0 5和 0 .0 1 )。上述指标的变化幅度随G值增大而增大。结论 +Gx负荷可引起脑能量代谢发生变化 ,这种变化可能是Objective To observe the changes of energy metabolism of brain tissue in rats under +Gx loads, and to explore its possible role in changes of brain function and work efficiency induced by +Gx stress. Method Forty five male Wistar rats were randomly divided into control, +5 Gx, +10 Gx, +15 Gx and +20 Gx group. Each group was exposed to the corresponding G value for 3 min. After that, cortical adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP) and lactic acid (LA) content, lactate dehydrogenase (LDH) activity were measured. Result Compared with the control group, the cortical LA content increased significantly after +5 Gx, +10 Gx, +15 Gx and +20 Gx exposure (P<0.01). Cortical ADP content and ratio of ADP/AMP and AMP/ATP increased significantly after +10 Gx, +15 Gx and +20 Gx exposure (P<0.01), whereas ATP content, energy charge and LDH activity decreased significantly (P<0.05 or 0.01). Cortical AMP content increased significantly after +15 Gx and +20 Gx exposure (P<0.05 and 0.01). Conclusion It is suggested that +Gx load can result in obvious depression of brain energy metabolism, which could be an important reason for the change of brain function and work efficiency induced by +Gx stress.

关 键 词：+GX 大鼠 脑组织 能量代谢 加速度应激 乳酸 乳酸脱氢酶 

分 类 号：R852.21[医药卫生—航空、航天与航海医学]