通痹灵对佐剂性关节炎大鼠滑膜细胞MCP-1 mRNA表达的影响  

Effect of Tongbiling on the Expression of MCP-1 mRNA of Synovial Cells in Rats with Adjuvant Arthritis

作　　者：熊茂林[1] 宋畅[1] 林敬明[2] 宋烈昌[2] 

机构地区：[1]中山大学中山医学院,广州510080 [2]第一军医大学珠江医院,广州510515

出　　处：《中药材》2002年第10期722-724,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:探讨中药复方制剂通痹灵(以桂枝芍药知母汤为基础)对佐剂性关节炎大鼠滑膜细胞单核细胞趋化蛋白 1(MCP-1)mRNA表达的影响。方法:采用半定量RT-PCR法测定佐剂性关节炎大鼠滑膜细胞MCP-1 mRNA表达水平。结果:通痹灵明显抑制了佐剂性关节炎大鼠滑膜细胞MCP-1 mRNA的表达,提示通痹灵治疗类风湿性关节炎的分子机制与抑制MCP-1基因表达有关。Objective: To study the effect of Tongbiling, a complex preparation of Chinese traditional medicine, on the expression of MCP-1 mRNA of synovial ecus in rats with adjuvant arthritis. Method: The expression level of MCP-1 mRNA in synoviocytes from rats with adjuvant arthritis was assayed by the method of RT-PCR. Result: The amounts of MCP-1 mRNA expression in synoviocytes from rats with adjuvant arthritis were distinctly decreased by Tongbiling. It suggested that Tongbiling had inhibition effect on rheumatoid arthritis by a down regulation for the expression of MCP-1 mRNA in the inflamed synoviocytes.

关 键 词：类风湿性关节炎 通痹灵 佐剂性关节炎 MCP-1 mRNA RT-PCR 动物实验 基因表达 

分 类 号：R259.9[医药卫生—中西医结合] R285.[医药卫生—中医内科学]