肥胖者外周脂肪组织中leptin受体水平的研究  

Leptin receptor in peripheral adipose tissues of obese subjects

作　　者：杜同信[1] 孙君江[1] 王自正[1] 王书奎[1] 傅雷[1] 韩流[1] 黄敏[2] 吴晓冬[2] 

机构地区：[1]南京医科大学南京市第一医院放免中心,210006 [2]中国药科大学

出　　处：《中华核医学杂志》2002年第5期309-310,共2页Chinese Journal of Nuclear Medicine

摘　　要：目的　研究肥胖者外周脂肪组织瘦素 (leptin)受体表达及探讨肥胖发生发展的机制。方法　用放射配基受体结合方法 ,检测 71例受检者 (其中肥胖 32例 ,超重 1 9例 ,正常对照 2 0例 )外周脂肪组织leptin受体密度。结果　随着体重指数 (BMI)的增加 ,肥胖组和超重组leptin受体密度与对照组比较差异有显著性 (P <0 0 1 ) ,肥胖组与超重组比较差异亦有显著性 (P <0 0 1 ) ;而受体与leptin的结合能力 (Kd 值 )差异无显著性 (P >0 0 5)。 3组间Kd 值差异无显著性 ,表明leptin受体与配基的结合能力与BMI无关。从散点分布图看 ,BMI越大其leptin受体密度越小 ,BMI与最大结合量相关 (r=- 0 76 ,P <0 0 1 )。结论　肥胖者外周脂肪组织中leptin受体的表达与BMI密切相关 ,而肥胖者血液中leptin水平升高 ,表明体内存在leptin受体水平下调致leptin耐受 ,继而形成肥胖。Objective To investigate the relationship between leptin receptor and obesity by studying the leptin receptor density B max and dissociation constantK d in peripheral adipose tissue in subjects with different body weight mass(BMI). Methods Leptin receptor density B max andK d were assayed via radioligand method in 71 cases, including 32 classified as obese, 19 over-weight and 20 normal controls Results With the escalating of BMI, the leptin receptor density significantly decreased in obese and over-weight group compared with that in normal controls (bothP<0 01), and there was also statistical difference between obese and over-weight group (P<0 01). TheK d values were of no differences among all three groups suggesting no correlation between the binding ability of leptin to its receptor and BMI A negative correlation between BMI and B max(r=-0 76,P<0 01) displayed after all Conclusion Leptin receptor density correlates with the BMI in obese cases and it suggests that the down-regulation of leptin receptor may contribute to the occurrence of leptin resistance and obesity afterwards.

关 键 词：外周脂肪组织 肥胖症 放射配位体测定 瘦素受体 相关性 

分 类 号：R589.2[医药卫生—内分泌]