硝酸稀土经乳汁对小鼠仔代免疫功能的影响  

The effects of exposure to rare earth(NO_3)_3 on the immune function of mice off spring via milk

作　　者：刘志宏[1] 雷志明[2] 魏雪涛[2] 薛彬[2] 

机构地区：[1]宁夏医学院预防医学系,银川750004 [2]北京大学医学部毒理系

出　　处：《中华预防医学杂志》2002年第6期394-397,共4页Chinese Journal of Preventive Medicine

摘　　要：目的　了解硝酸稀土能否经乳汁进入仔鼠体内及稀土对仔鼠免疫功能的影响。方法　待孕鼠生下仔鼠当天 ,分别经口灌胃给予 2、2 0、2 0 0mg/kg混合硝酸稀土溶液 (对照组给与蒸馏水 ) ,直至离乳。对刚离乳及离乳 3周仔鼠分别测定其免疫器官重量、脾抗体细胞数 (IgM PFC/脾 )、迟发型变态反应 (DTH)和巨噬细胞功能。结果　哺乳期母鼠经口灌胃混合硝酸稀土后 ,刚离乳仔鼠 2 0 0mg/kg体重比对照组下降了 18 8% ,2mg/kg组IgM PFC比对照组升高了 82 7% ,2 0mg/kg组仔鼠碳粒廓清速率κ值和廓清指数α值均明显高于对照组 ,各剂量组DTH与对照组相比差异均无显著性。离乳后3周仔鼠 2 0、2 0 0mg/kg组IgM PFC/脾分别比对照组降低了 4 7 0 %和 4 4 7% ,2 0 0mg/kg组碳粒廓清速率K值和廓清指数α值均明显低于对照组 ,各剂量组DTH与对照组相比差异均无显著性。结论　硝酸稀土对仔鼠免疫功能可产生一定的影响 ,且这些影响可能是硝酸稀土经乳汁进入仔鼠体内的结果。Objective To study the changes of immune function in mice offspring whose mothers were exposed to Rare Earth (RE)(NO 3) 3 Methods RE(NO 3) 3 was administered to mother mice after giving birth by gavage at dosages of 2, 20 and 200 mg/kg bw during breast feeding period The weights of spleen and thymus, the spleen plaque forming cells (PFC), the delayed type hypersensitivity (DTH) and the charcoal clearance of the offspring were determined Results The results obtained from the offspring after weaning showed that the body weight of offspring treated with 200 mg/kg RE(NO 3) 3 was 18 8% lower than that of the control group; at the dosage of 2 mg/kg, the number of IgM PFC was increased by 82 7%; and at the dosage of 20 mg/kg the rate of clearance and clearance index were significantly higher than that of the control group No difference in DTH was found in any treated group as compared to the control group The results of offspring at three weeks after weaning showed that the number of IgM PFC of the 20 and 200 mg/kg bw dose groups were 47 0% and 44 7% lower than that of control group respectively; the rate of clearance and clearance index of the 200 mg/kg group were significantly lower than that of the control group No significant changes in DTH were observed in each exposed group Conclusion RE(NO 3) 3 treatment affected the immune function of mice offspring which may caused by breast milk

关 键 词：硝酸稀土 乳汁 小鼠 仔代 免疫功能 

分 类 号：R114[医药卫生—卫生毒理学]