不同温度及时间对保存血液有效期和质量的影响  

EFFECTS OF DIFFERENT TEMPERATURE AND TIME ON THE PERIOD OF VALIDITY AND QUALITY IN BLOOD PRESERVATION

作　　者：杜静平[1] 邢天荣[1] 马奎力[1] 邢凤有[1] 

机构地区：[1]佳木斯大学医学院病理生理教研室,黑龙江佳木斯154002

出　　处：《中国应用生理学杂志》2002年第4期362-364,共3页Chinese Journal of Applied Physiology

摘　　要：目的 :研究不同温度对血液液状保存时保存损伤机制的影响 ,并探讨相应的防范措施。方法 :取 10名健康献血员静脉血 ,混合于CP2D A保存液中 ,均分为 2组 ,分别保存于 0℃和 4℃环境条件下 ,分别于设定的时间 (第2 1d和第 4 2d)以shafiq UR rehman法检测膜磷脂 ,同步法检测Na+ K+ ATP酶、纯化PDE法检测CaM、分光光度法检测LPO等指标。结果 :固定温度条件下随时间的延长血液过氧化反应增强 ,保存损伤作用增加 ;同一时期内保存损伤作用随温度的降低而减轻 ,以 4℃组血液老化明显。结论 :血液保存损伤作用随保存期的延长而增强 ,随保存温度的降低而改善 ,并指出血液液状保存所存在的问题与展望。Aim: To examine the effects of different temperature protection on measures on preservation damages in liquid blood and explore the corresponding. Methods: Take equal half blood samples from 10 healthy blood donors and devided each sample intotwo groups, put the fresh blood into CP2D A solution at 0℃ and 4℃, respectively and take the samples 21 days and 42 days, later and then measured the contents of membrane phospholipids with shafig UR rehman method, CaM with purification PED test, LPO with spectrophotometry. Results: At the same temperature, when the preservation time was prolonged, peroxidation was increased, the preservation damages were also augmented; the damages were declined when the temperature was lower during the same period, the aging of blood was more evident at 4℃. Conclusion: Blood peroxidation temperature is lower. The author pointed out the questions and prospects of blood preservation.

关 键 词：温度 时间 保存血液 有效期 质量 血流保存 保存损伤 

分 类 号：R457.12[医药卫生—治疗学]