猪肌肉蛋白质含量的QTL分析(英文)  

Detection of Quantitative Trait Loci for Protein Percentage in Muscle in Resource Pig Population

作　　者：姜勋平[1] 熊远著[1] 邓昌彦[1] 屈彦纯[1] 刘桂琼[2] 

机构地区：[1]华中农业大学农业部猪遗传育种重点开放实验室,武汉430070 [2]扬州大学畜牧兽医学院,扬州225009

出　　处：《Acta Genetica Sinica》2002年第12期1068-1072,共5页

基　　金："973"国家重大基础研究项目 (G2 0 0 0 0 1610 5 );国家高技术研究发展计划专项经费;博士点基金资助~~

摘　　要：用梅山猪与大白猪构建的资源家系检测影响肌肉蛋白质含量的数量性状座位 (QTL)。测定 135头F2 代个体及其相应父母和祖父母分布在 1号、2号和 6号染色体上的 2 4个微卫星基因型。用这些微卫星标记计算每个F2 代个体固定位置的加性和显性系数 ,以 1cM的间距将肌肉蛋白质含量与这些系数进行回归分析 ,发现 3个与肌肉蛋白质含量具有显著效应的QTL(P <0 .0 5 ) ,它们分别位于 1号染色体的Sw1332和Sw2 130之间、2号染色体的Sw2 5 16和Sw12 0 1之间、6号染色体的Sw2 5 16和Sw12 0 1之间 ,分别解释 0 .91%、12 .76 %和 4 .6 0 %的肌肉蛋白质含量变异方差。结果提示 ,影响肌肉蛋白质含量的QTL分布在多条染色体上。An intercross between Meishan and Large White pig population was used to map quantitative trait loci(QTL) for protein percentage in muscle. In the present study,all 135 F 2 animals,their parents and their grandparents were genotyped for 24 microsatellites,which were in 1,2 and 6 chromosome. The marker genotypes were used to calculate the additive and dominant coefficients at fixed position in the genome of each F 2 animal,and the protein percentage were regressed on to the coefficients in intervals of 1cM. Three significant QTLs effects were found for protein percentage in muscle. They were located between markers Sw1332 and Sw2130 at chromosome 1,between Sw2516 and Sw1201 at chromosome 2 and between Sw322 and Sw607 at chromosome 6( P <0.05),and the explained residual variance were 0.91%,12.76% and 4.60%,respectively. The results show that QTLs,which affect protein percentage,may be located in different chromosomes.

关 键 词：猪 肌肉蛋白质含量 QTL分析 肉质 

分 类 号：S828.2[农业科学—畜牧学] TS251[农业科学—畜牧兽医]