Efficiency of Artificial Insemination in Giant Pandas at the Wolong Breeding Center  

大熊猫人工授精的研究

作　　者：黄炎[1] 王鹏彦[1] 张贵权[1] 张和民[1] 李德生[1] 汤纯香[1] 魏荣平[1] 胡大明[1] 冯莉[1] Howard J G 

机构地区：[1]中国保护大熊猫研究中心,四川623006 [2]美国国家动物园,华盛顿特区200082598

出　　处：《Developmental and Reproductive Biology》2002年第2期118-125,共8页发育与生殖生物学报（英文版）

摘　　要：The goal of the giant panda ( Ailuropoda melanoleuca ) breeding program is to develop a self sustaining,genetically diverse population.Due to the common problems about sexual incompatibility and a limited number of captive born males that breed naturally,artificial insemination (AI) has become a critical genetic management tool.It is common practice,however,to combine natural mating and AI using semen from non breeding males.From 1998 to 2000 at the Wolong breeding facility,12 of 18 (66.7%) females produced 20 cubs following combined natural mating and AI.The objective of this study was to determine the efficiency of AI without natural breeding.In 1998 and 2000,seven females were anesthetized for transcervical AI on two consecutive days.Ejaculates from six males were collected by electroejaculation,diluted in an egg yolk diluent containing 0% or 4% glycerol and used either fresh or following cold storage at 4℃ (for 24 or 48 h) or cryopreservation using the pellet freezing method.Mean (±SEM) ejaculate traits in six male sperm donors were:ejaculate volume,3.3±0.5 ml;sperm concentration,1,429.8±235.4×10 6/ml;sperm motility,81.7±2.1%;progression (0～5,5=best),3.1±0.1;and normal sperm,79.3±9.2%.For AI (n = 14) in seven females,mean inseminate traits were:spermic volume inseminated,2.4±0.3 ml;sperm motility,73.5±2.9%;progression,2.5±0.1;and total motile sperm inseminated/AI,684.2±118.2×10 6.Four of seven (57.1%) females became pregnant and produced five cubs of which four survived.Mean gestation and litter size was 131.5±9.7 days and 1.3±0.3 cubs/litter,respectively.These results indicate that the efficiency of AI is sufficient for recovering valuable genes from non breeding individuals to enhance genetic diversity in the ex situ population of giant pandas.繁殖大熊猫的目的是圈养种群的自我维持和保持遗传多样性。由于圈养种群中有自然交配能力的雄兽很少 ,人工授精则成为有效的遗传管理的重要手段。本研究的目的是确定单纯人工授精的效率。 1 998年至 2 0 0 0年期间 ,在中国保护大熊猫研究中心 ,对 7只大熊猫进行了人工授精 (每只连续 2d) ,精液通过人工采精方法从 6只不同的雄兽中获得 ,使用鲜精、冷藏精液和冻精多种方法进行人工授精。 6只雄兽的精液平均值是 :采精量 3.3±0 .5ml;精子密度 1 ,42 9.8± 2 35 .4× 1 0 6/ml;活力 81 .7± 2 .1 % ;运动状态 ( 0～ 5 ,5 =最好 )3 1± 0 .1 ;精子正常率 79 3± 9.2 %。对 7只大熊猫进行的 1 4次人工授精中 ,使用的精液体积为 2 4± 0 3ml;活力是 73.5± 2 .9% ;运动状态为 2 .5± 0 .1 ;每次人工授精总活动精子数是 684.2± 1 1 8.2× 1 0 6。 7只大熊猫有 4只受孕 ( 5 7.1 % ) ,共产 5仔。平均孕娠期 1 31 .5±9.7d ,每胎平均 1 .3± 0 .3仔。同时 ,运用自然交配与人工授精相结合的方法 ,进行了 1 8只次实验 ,成功 1 2只次 ,产仔 2 0只 ,繁殖成功率为 66.7%。本研究结果表明 ,人工授精能有效地使不能自然交配的雄性大熊猫参与繁殖 ,提高繁殖率 ,增加圈养大熊猫的遗传多样性。

关 键 词：giant panda artificial insemination genetic phylogeny 

分 类 号：S865.31[农业科学—野生动物驯养]