电针穴位对脑缺血-再灌流大鼠海马和大脑皮层AChE活性的影响  

Influence of electroacupuncture points on AChE activity of hippocampus and cortex caused by cerebral ischemia-reperfusion rats

作　　者：柏志全[1] 周丽丽[1] 郑辉[1] 周卓妍[1] 王跃春[1] 林兴会[1] 姚平[1] 

机构地区：[1]暨南大学医学院生理学教研室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2002年第5期101-104,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省中医药管理局资助项目(344180)

摘　　要：　目的:探讨电针穴位对脑缺血-再灌流损伤引起的脑海马及大脑皮层内乙酰胆碱酯酶(AChE)活性的影响.方法:采用夹闭大鼠双侧颈总动脉(CCAO)造成脑缺血-再灌流损伤模型,以改良Ellman法测定AChE.每日电针双侧"百会"、"风池"、"大钟"及"足三里"穴30min、7和14d,疏-密波频率:2～20Hz,强度2 0A.结果:实验后第7d,大鼠海马及大脑皮层内的AChE活性在缺血-再灌流组明显低于假手术组,而其电针组的AChE活性则基本恢复;第14d,缺血-再灌流及其电针组的大脑皮层AChE活性恢复,海马AChE活性有所恢复.结论:脑缺血-再灌流所致的脑损伤可能与脑内AChE的活性有关;电针能提高大鼠海马及大脑皮层内的AChE活性,从而可能对抗脑缺血-再灌流所致的脑损伤作用.Aim:To investigate the influence electroacupuncture points on hippocampus and cortex acetylcholinesterase(AChE) activity of cerebral ischemia-reperfusion rats. Methods: Using animal models of cerebral ischemia-reperfusion-common carotid artery occlusion(CCAO), applying improved Ellman method to determine AChE activity and electroacupuncture bilateral points of 'Baihui?Fengchi?Dazhong? Zusanli' 30 min everyday and last 7 and 14 days, the frequency:2～20 Hz, the intensity: 2.0 A. Results: 7th day after experiment,in hippocampus and cortex, AChE activity of ischemia-reperfusion group was significantly decreased compared with sham-operation group,but electroacupuncture group was recovered. 14th day,AChE activity of hippocampus in ischemia-reperfusion group was lower than sham-operation, in electroacupuncture group, it was recovered too; But AChE activity of cortex, in ischemia-reperfusion group and electroacupuncture group had recovered to the level of sham-operation group. Conclusion: Decreasing of AChE activity in hippocampus and cortex may relate to cerebral ischemia -reperfusion injury; Electroacupuncture can improve the AChE activity.

关 键 词：电针穴位 大鼠 海马 大脑皮层 ACHE活性 电针治疗 脑缺血-再灌流 乙酰胆碱酯酶 脑损伤 

分 类 号：R651.15[医药卫生—外科学] R245[医药卫生—临床医学]