肺炎链球菌18C型糖蛋白结合物的制备及其免疫原性  

Preparation and immunogenicity of a conjugate composed of pneumococcal type 18C capsular polysaccharide bound to tetanus toxoid

作　　者：孙述学[1] 王建国[1] 刘方蕾[1] 王剑虹[1] 陈晓航[1] 王欣茹[1] 乔瑞洁[1] 陈美华[1] 

机构地区：[1]兰州生物制品研究所,兰州730046

出　　处：《微生物学免疫学进展》2002年第4期18-22,共5页Progress In Microbiology and Immunology

基　　金：甘肃省自然科学基金资助 ;ZS0 2 1-A2 5 -0 97-Y

摘　　要：制备肺炎链球菌 18C型荚膜多糖 破伤风类毒素结合物 (CPS TT) ,测定结合物的理化性质、抗原特异性及其在动物中的免疫原性。结果显示 ,结合物能与相应的多糖和破伤风抗血清形成明显的沉淀线 ,蛋白 /多糖比率为 1.86 ,结合物分子大小 (Kd值 )为 0 .0 5 8。注射小鼠后可诱导明显的抗体应答 ,而且随着注射针次的增加 ,抗体反应水平明显增高 ,显示加强效应。结果表明 ,制备的肺炎链球菌糖蛋白结合物抗原性良好 ,具有胸腺依赖性抗原的特性 。A conjugate of pneumococcal type 18C capsular polysaccharide(CPS) binding with tetanus toxoid (TT) was prepared by carbodiimide-mediated coupling of apidic acid hydrazide (ADH)derivative.The physico-chemical property, antigen specificity and immunogenicity of conjugate have been investigated. The results showed the CPS-TT can produce visible precipitation lines with antisera of pneumococcal 18C type polysaccharide and tetanus toxoid respectively.The ration of proteine and polysaccaride is 1.86,molecular size of conjugate is 0.058(Kd value),it can induce higher levels of specific serum lgG antibody responses in mice. Furthermore along with the increasing of injection doses, the antibody concentration are significantly rising(P=0.01) to show the boosed effection. In summary, the prepared pneumococcal conjugate showed satisfactory immugenicity in mice and maintained perfect antigenicity, it has the property of thymus dependent antigen.

关 键 词：肺炎链球菌 18C型糖蛋白结合物 制备 免疫原性 荚膜多糖 

分 类 号：R392[医药卫生—免疫学]