A群脑膜炎球菌多糖-破伤风类毒素结合物中游离多糖测定方法的建立  

A method for quantitative test of unconjugated meningoccocal polysaccharide(PS) in PS-tetanus toxoid conjugate preparations

作　　者：朱为[1] 余生玲[1] 朱文瑛[1] 王晓[1] 黄帼英[1] 李凤祥[2] 李亚楠[2] 

机构地区：[1]上海生物制品研究所,上海200052 [2]中国药品生物制品检定所

出　　处：《微生物学免疫学进展》2002年第4期29-32,共4页Progress In Microbiology and Immunology

摘　　要：本文建立一种测定A群脑膜炎球菌多糖 破伤风类毒素 (PS TT)结合物中游离多糖的方法。用 80 %乙醇使PS TT结合物沉淀 ,再用 6 0 %乙醇洗涤沉淀使未结合多糖溶解 ,使与TT结合的和未结合的多糖分离并分别测定。该方法可有效分离结合的与未结合的多糖并分别测定 ,结合物中外加多糖可定量回收。本方法适用于测定A群脑膜炎球菌多糖The method for quantitative test of free meningoccocal polysaccharide(PS) in PS-tetanus toxoid conjugate was developed based on the ethanol precipitation of tetanus toxoid(TT).The conjugate was treated with 80% ethanol to precipitate PS-TT, then the precipitant was washed with 60% ethanol to resolve free PS, and to separate unconjugated PS from conjugated PS-TT.This method could separate free PS from conjugated PS-TT effectively and quantitative.The accuracy of the assay was confirmed by spiking known amounts of free PS to PS-TT conjugate preparations. This method is useful for measuring content of unconjugated PS in preparations of meningoccocal PS-TT conjugate.

关 键 词：破伤风类毒素 测定方法 A群脑膜炎球菌 多糖结合物 游离多糖 

分 类 号：R392[医药卫生—免疫学]