登革病毒基因组核苷酸序列与血清学分型的相关性研究  

The Relationships of Serotypes and Nucleotide Sequences of The Dengue viruses Genome

作　　者：宋宏[1] 付士红[1] 王寰宇[1] 梁国栋[1] 

机构地区：[1]中国预防医学科学院病毒学研究所病毒基因工程国家重点实验室,北京100052

出　　处：《中国病毒学》2002年第4期297-303,共7页Virologica Sinica

基　　金：国家自然科学基金资助 (3 0 170 0 46)

摘　　要：为证明登革病毒基因组核苷酸序列与病毒血清学分型的相关性 ,本文以病毒全基因组和病毒 5′末端非编码区 ,E蛋白 ,NS1蛋白和 3′末端非编码区四个基因区段分别进行病毒型间比较。比较结果表明病毒全基因组和病毒的各个基因区段均明显分为相互独立的 4个分支 ,与病毒血清型分型完全吻合 ,不存在型间重组的现象。The aim of this study was to prove the relationships of serotypes and nucleotide sequences of the Dengue viruses genome. To this end, the nucleotide sequences of 5′ noncoding region,3′ noncoding region,envelope E glycoprotein,nonstructural protein NS1 were aligned correspondingly. With four Dengue viruses phylogenetic groups which were corresponding to four serotypes each other being identified, Our results demonstrate that there were no intergenomic recombination observed. The possibility of intragenomic recombination and the similarity of nucleotide sequences of different Dengue viruses were analysed.

关 键 词：登革病毒 基因组 核苷酸序列 血清学分型 相关性 

分 类 号：R346[医药卫生—基础医学]