利用杆状病毒表达幽门螺杆菌cagA基因  

Expression of Helicobacter pylori cagA gene in Insect Cells by Baculovirus

作　　者：梁钧[1] 龚岷[1] 袁志明[1] 梁布锋[1] 

机构地区：[1]中国科学院武汉病毒研究所,湖北武汉430071

出　　处：《中国病毒学》2002年第4期336-339,共4页Virologica Sinica

摘　　要：幽门螺杆菌cagA基因克隆到杆状病毒表达系统的 pBlueBacHis2A转移载体中 ,将重组质粒 pBlueBacHis2A CagA与亲本病毒Bac N blueDNA共转染Sf9细胞 ,以空斑法纯化获得的重组杆状病毒。经PCR法鉴定后进行扩增培养 ,SDS PAGE和Westernbolt检测结果证实所表达的蛋白为CagA蛋白 ,间接ELISA分析表明 。A recombination transposing vector pBlueBacHis2 CagA was constructed by inserting \%Helicobacter pylori\% cagA gene into pBlueBacHis2A vector of baculovirus expession system. Co transfecting Sf9 cells with Bac N blue DNA and pBlueBacHis2 CagA, recombinant viral DNA was obtained by purification of recombinant plaques.PCR result showed cagA gene intergrating in correct position of baculovirus gemone. The expression of Helicobacter pylori cagA gene was confirmed by SDS PAGE and Westernbolt. Using indirect ELISA assay, it was found that the protein exhibited good specific reactivity with sera of HP infected individuals.

关 键 词：杆状病毒 表达 幽门螺杆菌 CAGA基因 昆虫细胞 

分 类 号：R346[医药卫生—基础医学]