大肠杆菌tktA基因的克隆表达  

Cloning and expression of transketolase A gene of E.coli

作　　者：李永辉[1] 刘云[1] 王世春[1] 张学清[1] 宋文全[1] 程莉[1] 徐琪寿[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《生物技术通讯》2002年第6期427-429,共3页Letters in Biotechnology

摘　　要：tktA是芳香族氨基酸生物合成共同途径的关键酶基因之一,在大肠杆菌中,tktA编码转酮酶A,在磷酸戊糖途径生成4-磷酸赤藓糖中起主要作用。采用PCR方法从大肠杆菌K-12株中扩增到tktA,并实现了高效表达,TktA活性提高了3.9倍,并且使芳香族氨基酸生物合成共同途径中关键中间产物DAHP的产量有所提高。tktA is the key gene in central metabolism of aromatic amino acids biosynthesis,and encoding transketo-lase A in E.coli.It plays a major role in erythrose-4-phosphate(E4P)production of pentose pathway.We amplified tktA from E.coli K-12by PCR and expressed it in E.coli DH5α.The SDS-PAGE demonstrated that the band at73KD was more intensive than the same one of the control.The specific activity of TktA in crude extracts was in-creased by3.9-fold.It promoted the production of E4P,the first intermediate of the common aromatic pathway.

关 键 词：大肠杆菌 tktA基因 克隆 表达 芳香族氨基酸 生物合成 代谢工程 

分 类 号：Q785[生物学—分子生物学] Q786