金属硫蛋白与皮瓣缺血预处理延迟保护作用的实验研究  

The effect of metallothionein on delaying protection of ischemia/reperfusion

作　　者：郭杰[1] 姜洪池[1] 王淑杰[1] 

机构地区：[1]哈尔滨医科大学附属第二医院整形外科

出　　处：《中华整形外科杂志》2002年第6期350-352,共3页Chinese Journal of Plastic Surgery

摘　　要：目的　探讨金属硫蛋白 (MT)参与皮瓣缺血预处理 (PC)延迟保护作用 (DP)的可能性。方法　在猪背阔肌岛状皮瓣PC和培养肌细胞PC的模型上 ,检测PC后即刻 ,12h和 2 4hMT含量变化 ,观察PC后 2 4h对再次长时间缺血再灌注 (I R)或缺氧复氧 (A R)损伤的保护作用以及用丝裂素蛋白激酶抑制剂 (PD0 980 59)抑制PC后MT含量增高对PC和DP的影响。结果　MT含量在PC后 12h(肌细胞 )和 2 4h(肌细胞和皮瓣组织 )显著增高 ,与未PC的皮瓣组织或肌细胞遭受I R或A R的损伤相比较 ,PC后 2 4h其皮瓣坏死范围缩小 (P <0 0 5 ) ,血浆乳酸脱氢酶 (LDH)活性降低 (P <0 0 5 ) ,肌细胞存活率增高 ,细胞丙二醛 (MDA)含量和LDH释放均降低 (P <0 0 1)。用PD0 980 59抑制后则消除了PC后的DP作用 ,上述皮瓣损伤指标接近单纯I R组或A R组 (P >0 0 5 )。结论　①PC后 2 4h对皮瓣或肌细胞的再次I R或A R损伤具有保护作用 ;②MT参与了皮瓣或肌细胞PC后的DP作用。Objective To study the possibility of metallothionein (MT) participating in the process of ischemia/reperfusion protection. Methods The model of pig latissimus dorsi island flap and cultured myocytes were used in this study. The island flap was preconditioned with ischemia and the cultured myocytes were treated with anoxia. MT was detected in the flap and the cultured myocytes instantly and at 12 and 24 hours after the treatment. The protection effect of precondition on flap ischemia/ reperfusion damage was observed by recording flap necrosis and the serum LDH level. The protection effect of precondition on myocyte anoxia/reoxygenation damage was observed by recording the cell survival rate and MDA content. The change in the protection effect was also observed after the MT was inhibited by PD 098059. Results The content of MT in cultured myocytes increased obviously 12 hours after precondition. Similar result was observed in flap tissue 24 hours later. Compared with the non-conditioned flap, the necrosis areas of the preconditioned flaps were smaller, the serum LDH was lower. The survival rate of preconditioned cultured myocytes was higher compared with non-conditioned cells (P<0.05). The content of MDA and the quantity of discharged LDH were less (P<0.05). When MT was inhibited by PD 098059, the delaying protection of precondition disappeared. The observed quotas at that time were the same with those of simple I/R or A/R groups (P>0.05). Conclusion 24 hours after precondition, the flap and the cultured myocytes are protected from re-damage of I/R or A/R. MT is involved in this process.

关 键 词：金属硫蛋白 缺血预处理 延迟保护作用 缺血再灌注 整形外科 

分 类 号：R622[医药卫生—整形外科]