柞蚕微孢子虫孢子人工发芽的研究  

A Study on Artificial Induction of Nosema pernyi Spores

作　　者：王爱荣[1] 李健男[1] 刘向国[2] 夏润玺[1] 冀瑞琴[1] 

机构地区：[1]沈阳农业大学,辽宁沈阳110161 [2]福建农业大学,福建福州350002

出　　处：《沈阳农业大学学报》2002年第4期281-284,共4页Journal of Shenyang Agricultural University

摘　　要：采用6种人工发芽方法研究了柞蚕孢子虫孢子发芽条件。试验发现 ,柞蚕微孢子虫孢子的发芽率存在较大差异 ,其中KHCO3+K2CO3 法发芽率最高 ,达78.86%。在柞蚕微孢子虫孢子的人工发芽过程中 ,K+ 和Na+ 对其有促进作用 ,并且K+ 对柞蚕微孢子虫孢子发芽的促进作用明显强于Na+。Ca2 +和Mg2 +则对孢子发芽有显著阻碍作用 ,CO32 -有协同促进作用。而且 ,还证实柞蚕微孢子虫孢子在人工发芽过程中的完全发芽时间为10min。经固定染色后发现孢子发芽前后的形态发生变化 ,并能观察到弹出的极丝。This study demonstrated that there was a great difference in germinating rates by different induction ways and the germinating rates were different in different germination media with the same artificial induction method. K+ and Na+enhanced hatching.Synergisty of CO32- to K+ and Na+ appeared. Moreover, the prometing effect of K+ in artificial induction of N.pernyi spores was much notable than that of Na+. Ca2+ and Mg2+ inhibited the hatching of N.pernyi spores. It testified that perfect germinating of N. pernyi spores in artificial induction needed 10 minutes. After fixed and stained, the shape of hatched spores changed compaired with that of unhatched spores and the long pole which extended out could be found.

关 键 词：柞蚕 微孢子虫 人工发芽 微粒子病 孢子发芽率 

分 类 号：S885.142.1[农业科学—特种经济动物饲养]