导入外源DNA大豆后代的抗虫性鉴定与筛选  

SELECTING AND EVALUATION OF MUTANT LINES RESISTANT TO INSECT PEST BY INDRODUCING EXOGENOUS DNA INTO SOYBEAN

作　　者：刘德璞[1] 袁鹰[1] 王成武[1] 周正平[1] 郑培和[1] 

机构地区：[1]吉林省农业科学院,公主岭136100

出　　处：《大豆科学》2002年第4期245-249,共5页Soybean Science

基　　金：吉林省科技发展计划项目。

摘　　要：大豆食心虫 (Leguwinivoraglycinivorella )和大豆蚜虫 (Aphidglycines)是东北大豆生产的主要虫害。为拓宽资源的利用 ,创造抗虫大豆种质 ,用花粉管通道技术向大豆品种吉 2 0、吉 2 5、吉 2 7、吉 30等导入皂角 (Gleditisiajaponica )、鹰嘴豆 (Cicerarietinum )和农家早期品种DNA ,对从后代中选出的变异系进行了多年抗虫性鉴定和筛选 ,得到了抗虫品系 4个。本文报告了抗虫鉴定筛选结果。Leguminivora glycinivorella and aphid glycines are major insect pest of soybean in northeastern area of China. In order to broaden applying of germplasm resource and create new variety resisting to insect pest, exogenous DNA form Gleditisia japonica,cicer arietinum and land race were introduced into soybean cultivars of Jilin 20,Jilin 25,Jilin 27 and Jilin 30 by pollen tube pathway technique. Four lines resistant to the insects ( leguminivora glycinivorella or aphid glycines ) were gained from the mutants through appraising test for 3-6 years. This paper report the results of selecting and appraising test.

关 键 词：外源DNA 抗虫性 鉴定 筛选 大豆 抗虫育种 食心虫 蚜虫 转基因育种 花粉管通道技术 

分 类 号：S565.1[农业科学—作物学]