aFGF基因转染改善猪慢性缺血心肌心功能的实验研究  

IMPROVEMENT OF CARDIAC FUNCTION AFTER ADENOVIRUS-MEDIATED GENE TRANSFER OF ACIDIC FIBROBLAST GROWTH FACTOR TO CHRONIC ISCHEMIC MYOCARDIUM IN SWINE

作　　者：顾俊彦[1] 梅举[1] 黄盛东[1] 徐静[1] 张宝仁[1] 朱泉芳[1] 李白翎[1] 

机构地区：[1]第二军医大学长海医院

出　　处：《解放军医学杂志》2002年第11期979-980,共2页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 39970 35)

摘　　要：为探讨携带人酸性成纤维细胞生长因子 (aFGF)基因的重组缺陷型腺病毒载体 (Ad .aFGF)转染对缺血心肌心功能改善的作用 ,采用小型猪 ,开胸后于左回旋支起始部放置Ameroid环 ,4周后 ,Ad.aFGF(n =7)、Ad.Null(n =5 )、PBS(n =6 )直接注射到左回旋支供血区域心肌 ,每只注射 10点 ,每点注射病毒 10 9pfu或PBS 10 0 μl;注药后 4周进行心脏超声检测。结果显示 ,Ad.aFGF组动物心功能较Ad.Null组和PBS组有显著改善。研究表明 ,Ad.aFGF能改善缺血心肌的心功能 ,可用于缺血性心脏病的治疗。It has been reported that angiogenic growth factors may be useful in the treatment of ischemic heart disease. In our experiment a recombinant deficient adenovirus vector coding for aFGF was intramyocardially administrated into swines with chronic ischemic myocardium to investigate its effect on the improvement of myocardial function. In the present study, experimental minipigs underwent thoracotomy and placement of an Ameroid constrictor on the left circumflex branch coronary artery. Four weeks later, Ad.aFGF( n =7), Ad.Null( n =5) or PBS( n =6), was administrated directly into the myocardium at 10 sites in the circumflex branch distribution area (10 9 pfu or 100μl). Another four weeks later, cardiac function was examined by echocardiography and the results showed significant improvement of myocardial function in Ad.aFGF animals compared with Ad.Null and PBS animals. So such a strategy of gene therapy can be used in patients with ischemic heart disease.

关 键 词：基因转染 酸性成纤维细胞生长因子 病理性新生血管化 腺病毒 心肌缺血 心功能 基因治疗 心肌再血管化 

分 类 号：R541.4[医药卫生—心血管疾病]