胞内cAMP浓度的增加可诱导骨髓间质干细胞分化为神经元样细胞  

Human mesenchymal stem cells differentiate into neuron-like cells by increase in intracellular cyclic AMP

作　　者：项鹏[1] 夏文杰[1] 张丽蓉[2] 陈振光[3] 张秀明[1] 李艳[1] 李树浓[1] 

机构地区：[1]中山医科大学病理生理学教研室,广东广州510080 [2]广东药学院病理生理学教研室,广东广州510224 [3]中山医科大学附属第一医院心胸外科,广东广州510089

出　　处：《中国病理生理杂志》2002年第11期1324-1327,I001,共5页Chinese Journal of Pathophysiology

基　　金：国家重点基础研究发展规划项目 (973) (No.G1 9990 5430 0 )

摘　　要：目的 :探讨胞内cAMP浓度的增加与骨髓间质干细胞 (MSC)分化为神经元样细胞的关系。方法 :采用Ficoll-Paque液 (1 0 77g/L)离心分离成人MSC ,体外扩增 ,采用含腺苷酸环化酶激动剂Forskolin及磷脂酶抑制剂 3-异丁基 - 1 -甲基黄嘌呤 (IBMX)的无血清DMEM诱导MSC分化为神经元。免疫组化鉴定神经元烯醇化酶 (NSE)、神经丝蛋白 (NF)、胶质纤维酸性蛋白 (GFAP)的表达。结果 :成人骨髓间质干细胞在体外扩增原代可获得 5× 1 0 5,1 0代可获得 2× 1 0 1 0 个细胞。加入Forskolin、IBMX以及两者联用诱导后 ,MSC胞体收缩 ,突起伸出 ,呈典型神经元样形态 ;免疫组化显示诱导出的神经元样细胞NSE、NF -M表达阳性 ,GFAP阴性。结论AIM: To investigate the differentiation from human mesenchymal stem cells(hMSC) into neuron-like cells by the increase in intracellular cyclic AMP. METHODS: hMSC were separated from human marrow with Ficoll-Paque reagent and expanded in culture medium. hMSC were induced to differentiate into neurons with Forskolin and 3-isobutyl-1-methyl-xanthine (IBMX). Neuron-specific enolase(NSE), neurofilament(NF), glial fibrillary acaidic protein(GFAP) were detected by immunohistochemistry. RESULTS: hMSC were expanded as undifferentiated cells in culture for more than 10 passages. Forskolin/IBMX can induce hMSC to exhibit a neuronal phenotype, expressing NSE and NF-M in 5 hours. But the neuron-like cells didn't express the glial astrocyte marker GFAP. CONCLUSION: hMSC can be induced to differentiate into neurons by increase in the intracellular cAMP.

关 键 词：胞内cAMP浓度 骨髓间质干细胞 神经元样细胞 腺苷环-磷酸 福司柯林 诱导分化 

分 类 号：R338.1[医药卫生—人体生理学]