高果糖诱导的胰岛素抵抗大鼠外周血白细胞iNOS mRNA与ecNOS mRNA表达的变化  

The alteration of expression of iNOS mRNA and ecNOS mRNA in peripheral leukocytes from insulin resistance rats fed with fructose

作　　者：李瑞峰[1] 郭成浩[1] 魏树珍[2] 陈福琴[3] 陈融[1] 李莉[1] 刘玉梅[1] 胡维诚[1] 

机构地区：[1]山东大学医学院病理生理学教研室,山东济南250012 [2]济南市中心医院,山东济南250013 [3]山东大学齐鲁医院,山东济南250012

出　　处：《中国病理生理杂志》2002年第11期1404-1406,I004,共4页Chinese Journal of Pathophysiology

基　　金：山东省卫生厅资助项目 (99CA1CAA1 1 )

摘　　要：目的 :探讨高果糖饮食对Wistar大鼠外周血白细胞iNOSmRNA与ecNOSmRNA表达的影响。方法 :普通饲料喂养 ,实验组饮用 1 2 %的果糖水造型。于实验前、实验过程中 1 - 6个月每月断尾取血 ,测定外周血白细胞诱导型一氧化氮合酶mRNA(iNOSmRNA)与内皮源型一氧化氮合酶mRNA (ecNOSmRNA)表达 ;观察实验前、造型 1、2、3及 6个月时的空腹血糖、血浆胰岛素水平变化。结果 :1个月后 ,血糖与实验前无显著差异 ;血浆胰岛素明显高于对照组 (P <0 0 1 ) ,并持续维持在高水平上 ;外周血白细胞ecNOSmRNA表达增强。 2个月后血糖高于对照组 (P <0 0 5) ;外周血白细胞出现iNOSmRNA表达。 3及 6个月 ,血糖明显高于对照组 (P <0 0 1 ) ;iNOSmRNA与ecNOSmR NA持续保持高表达状态。结论 :高果糖饮食两个月 ,可引起Wistar大鼠产生明显的高胰岛素血症和胰岛素抵抗 ;首先ecNOSmRNA表达增强 ,可能延缓胰岛素抵抗的形成。继之 ,iNOSmRNA表达增强 。AIM: To study the alteration of expression of iNOS mRNA and ecNOS mRNA in peripheral leukocytes of Wistar rats fed with fructose. METHODS: Wistar rats were randomly divided into the control group ( n =10) and the fructose feeding group( n =10). The fructose feeding group drank 12% fructose water for 6 months. The blood glucose, blood insulin, and the expression of iNOS mRNA and ecNOS mRNA in peripheral leukocytes of rats were determined. RESULTS: The levels of blood insulin ( P< 0.01) and the expression of ecNOS mRNA were higher in fructose feeding group than that in control group after 1 month. The level of blood insulin( P< 0.01), the level of blood glucose ( P< 0.05), the expression of ecNOS mRNA and the iNOS mRNA were also higher in fructose feeding group than that in control group after 2 months. The levels of blood insulin and glucose, the expression of ecNOS mRNA and the iNOS mRNA were increased persistently during 3 to 6 months. CONCLUSIONS: These results indicate that fructose can increase the level, but reduce the sensitivity of insulin. It can also induce the expression of ecNOS mRNA firstly and the expression of iNOS mRNA secondly, the former can delay the formation of insulin resistance and the later can accelerate the formation of insulin resistance.

关 键 词：高果糖 胰岛素抵抗 大鼠 外周血白细胞 Ⅱ型糖尿病 INOS MRNA ecNOS MRNA 

分 类 号：R587.1[医药卫生—内分泌]