抗氧化剂预防低血糖所致肌肉损伤的研究  

Antioxidant prevents muscle damages induced by experimental hypoglycemia

作　　者：江钟立[1] 陈家伟[2] 周士枋[1] 励建安[1] 上月正博[3] 佐藤德太郎[3] 

机构地区：[1]南京医科大学第一临床医学院康复医学科,南京210029 [2]南京医科大学第一临床医学院内分泌科,南京210029 [3]日本东北大学医学部

出　　处：《中华物理医学与康复杂志》2002年第3期152-155,共4页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的　探讨低血糖状态下血浆和组织中谷胱甘肽含量的变化以及还原型谷胱甘肽 (GSH)对血清酶活性的抑制作用。方法　静脉注射胰岛素诱发低血糖 ,并持续 60min ,然后输注葡萄糖解除低血糖。结果发现诱发低血糖后 6h ,血浆GSH、氧化型谷胱甘肽 (GSSG)、谷胱甘肽总量 (TGSH)及GSSG/TGSH比值均明显升高 ,肝脏GSH和TGSH明显减少 ,心肌和骨骼肌GSSG和GSSG/TGSH比值明显增高 ,同时伴有血清酶 (ALT、AST、LDH、CK)活性升高。预先注射GSH后诱发低血糖组 ,血浆GSSG和GSSG/TGSH比值明显下降 ,并抑制了血清酶的活性。结论　低血糖所致的血清酶活性的升高与体内谷胱甘肽氧化还原状态的改变有关 。Objective To investigate the effect of hypoglycemia on glutathione levels in plasma and tissues and preventive effect of reduced glutathione (GSH) on serum enzymes. Methods In the model of hypoglycemia, changes in plasma and tissue contents of glutathione and inhibitory effects of reduced glutathione (GSH) on serum enzymes including alanine aminotransferase (ALT), aspartate aminotrasferase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK) were observed in the study. Results Besides the increase of serum enzymes (ALT, AST, LDH, CK), plasma GSH, oxidized glutathione (GSSG), total glutathione (TGSH) and GSSG/TGSH ratio rose significantly ( P <0.05) at 6 hours after the induction of hypoglycemia. Significant decrease in contents of GSH and TGSH in liver and significant increases in GSSG contents and GSSG/TGSH ratio in myocardium and quadriceps were observed during hypoglycemia in contrast with control group. Administration of GSH significantly decreased the plasma GSSG levels and GSSG/TGSH ratio ( P <0.05), and suppressed the increases of serum enzymes induced by hypoglycemia. Conclusion The results suggest that increase of serum enzymes induced by hypoglycemia might be related to change of redox state of glutathione and that administration GSH may play a preventive role of muscle from damage.

关 键 词：预防 肌肉损伤 低血糖症 抗氧化药 血清酶 还原型谷胱甘肽 

分 类 号：R587.3[医药卫生—内分泌]