不同激素处理对红火球紫薇组织培养的影响  

Effects of Different Hormones Treatment on Tissue Culture of Lagerstroemia indica 'Dynamite'

作　　者：鲁好君 崔媛媛[1] 白红娟[1] 邓小梅[1] 

机构地区：[1]华南农业大学林学与风景园林学院广东省森林植物种质创新与应用重点实验室,广东广州510642

出　　处：《广东林业科技》2015年第6期52-56,共5页Forestry Science and Technology of Guangdong Province

基　　金：国家林业局林业公益性行业科研专项"红叶紫薇组培快繁技术研究"(编号:201404116)

摘　　要：以红火球紫薇(Lagerstroemia indica‘Dynamite’)优树嫩枝为外植体,研究了不同浓度激素组合对红火球紫薇组培苗增殖及生根的影响。结果表明,最适腋芽诱导培养基为MS+6-BA 0.5 mg·L-1+IBA 0.05 mg·L-1,诱导率达72%。最佳增殖培养基为改良MS+6-BA 0.3 mg·L-1+IBA 0.02 mg·L-1,增殖系数可达4.6,芽高为3.8 cm;生根最适培养基为改良WPM+IBA 0.4 mg·L-1,生根率为98.5%,平均每株苗生根3~4条。炼苗后,移栽于泥炭土+珍珠岩(3:1,V/V)混合基质中,成活率为90%以上。Tissue cultural techniques of Lagerstroemia indica ‘Dynamite'were studied. The results showed that,MS + 6- BA 0. 5 mg ·L- 1+ IBA 0. 05 mg ·L- 1was optimal medium for induction,the induction rate of which reached 72%. The best mutiplication medium was modified MS + 6- BA 0. 3 mg·L- 1+ IBA 0. 02 mg·L- 1,the highest multiplication rate was 4. 6 and the height of axillary bud was 3. 8 cm. The most suitable rooting medium was modified WPM + IBA 0. 4 mg·L- 1. The rooting rate was 98. 5% and each seedling could take 3 to 4roots. The transplanting matrix were peat and pearlite( 3: 1,V / V),more than 90% rooted shoots survived transfer to the greenhouse.

关 键 词：红火球紫薇 组织培养 增殖培养 生根培养 

分 类 号：S685.99[农业科学—观赏园艺]