重组人IL-10抑制TNF-α和PDGF-BB所致大鼠血管平滑肌细胞增殖  

Recombinant human interleukin-10 inhibits vascular smooth muscle cell proliferation by TNF-α and PDGF-BB in vitro

作　　者：欧阳平[1] 彭立胜[1] 杨红[1] 吴文言[1] 彭文烈[1] 徐安龙△ 

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《中国病理生理杂志》2002年第12期1490-1493,共4页Chinese Journal of Pathophysiology

基　　金：广州市科技攻关项目 (No .JB0 0 0 0 0 4 4 81 65)

摘　　要：目的 :观察重组人白介素 1 0 (rhIL - 1 0 )对SD大鼠血管平滑肌细胞及血管损伤后新生内膜增殖的影响。方法 :培养大鼠主动脉血管平滑肌细胞 ,采用MTS/PES法确定血管平滑肌细胞的增殖状态 ,应用流式细胞术测定细胞周期。利用大鼠血管损伤模型 ,观察rhIL - 1 0对新生内膜增殖的影响。结果 :与对照组相比 ,rhIL - 1 0单独应用对血管平滑肌细胞生长没有影响 (P >0 0 5)。rhIL - 1 0可抑制在TNF -α和血小板源性生长因子分别刺激下的血管平滑肌细胞的生长 (P <0 0 5)。流式细胞术测定的结果显示rhIL - 1 0分别可以使TNF -α和PDGF -BB作用下的VSMC大部分处于G0 /G1 期 ,与对照组相比有明显差异 (P <0 0 1 )。大鼠颈动脉损伤后 ,rhIL - 1 0治疗组的动脉血管新生内膜 /中层面积比低于对照组约 45 % (P <0 0 1 )。结论 :抗炎细胞因子rhIL - 1 0可抑制大鼠血管平滑肌细胞的增殖。AIM: To determine the effects of recombinant human interleukin-10(rhIL-10) on proliferation of vascular smooth muscle cells(VSMC) by TNF-α and PDGF-BB and neointimal hyperplasia after rat carotid arterial injury.METHODS: Rat aortic VSMC was cultured and treated with rhIL-10 with or without tumor necrosis factor-α(TNF-α) and platelet-derived growth factor-BB (PDGF-BB), respectively.Proliferation of VSMC was quantified by colormetric assay.Cell cycle analysis was performed by flow cytomertry.SD rats were treated with recombinant human IL-10(rhIL-10) for 3 days after carotid arteries injury.Neointima to media area ratio at the site of arterial injury was measured at 28 days after balloon injury.RESULTS: Compared to control,both TNF-α and PDGF-BB stimulated VSMC proliferation. rhIL-10 alone had no effect on VSMC growth.With TNF-α or PDGF-BB stimulation,rhIL-10,at dose as low as 10 μg/L,inhibited VSMC growth( P <0 05) for both cases.Cell number in G 0/G 1 phase of PDGF-BB and rhIL-10 co-treatment group was higher than those of PDGF-BB treatment alone( P <0 01) by flow cytometry analysis.The same results were observed in TNF-α and rhIL-10 co-treatment group( P <0 01).Compared with the arterial injury group,neotima/media area ratio of recombinant human IL-10 group was reduced at 45%( P <0 01).CONCLUSIONS: The anti-inflammatory cytokine rhIL-10 inhibits TNF-α and PDGF-BB-induced VSMC proliferation,respectively.These results suggest the possibility that recombinant human IL-10 as a potential therapeutic approach prevents neointimal hyperplasia.

关 键 词：重组人IL-10 抑制 TNF-Α PDGF-BB 大鼠 血管平滑肌 细胞增殖 

分 类 号：R543.1[医药卫生—心血管疾病]