1株磷细菌基本培养条件的研究  

Culture condition of phosphate-solubilizing bacteria Bacillus megaterium var.phosphaticum

作　　者：梁锦锋[1] 陈欣[2] 唐建军[2] 

机构地区：[1]浙江林学院生命科学学院,浙江临安311300 [2]浙江大学生命科学学院,浙江杭州310029

出　　处：《浙江林学院学报》2002年第4期342-345,共4页Journal of Zhejiang Forestry College

基　　金：国家自然科学基金资助项目(39870149)

摘　　要：以磷细菌菌株巨大芽孢杆菌为对象,采用液体培养研究了各种理化因素对其生长的影响。结果表明:①适宜的pH值为5 5～9 5,最适pH范围为8 5～9 0。②在100mL三角瓶中,其最适装量为15～20mL;在装量大于25mL时,菌体生长明显受抑制。③菌体最大生长量时的温度为30℃左右。④作为菌体生长的碳源,葡萄糖最佳,半乳糖和甘露糖次之;单糖对于菌体生长的促进优于二糖,多糖最差。⑤Mg2+,Mn2+,Ca2+可以明显提高磷细菌在液体培养基中的初始生长量,Fe2+,Al3+等离子特别是Fe2+却对菌体的初始生长量有明显抑制作用。K+,Zn2+对于菌体初始生长影响不明显。Influence of different physical and chemical factors on the growth of phosphatesolubilizing bacteria(PSB) were investigated.The results showed that ①PSB was able to thrive and grow very well when its volume in a 100 mL flask was 20 mL,which was considered as optimal.②The bacteria grew well in slightly alkaline conditions and the optimal pH was 85～90.③The temperature for the largest number of cell after two days incubation was 30℃.④Glucose was the best source of carbon;the most suitable Nitrogen and Carbon concentrations were 8 g·L-1 and 021 g·L-1 respectively.⑤Mg2+,Mn2+,and Ca2+ could improve the quantity of the initial growth of this bacteria.But Fe2+ and Al3+ especially Fe2+ inhibit the bacterias initial growth.

关 键 词：磷细菌 培养条件 生态学 资源学 生长量 

分 类 号：S718.83[农业科学—林学]