大鼠成骨细胞中蛋白激酶A的表达及信号作用  

Expression and signal significance of protein kinase A on osteoblast cells in rats

作　　者：葛成[1] 杨连甲[2] 刘宝林[1] 赵丽君[1] 

机构地区：[1]第四军医大学口腔医学院颌面外科,陕西西安710032 [2]第四军医大学口腔医学院病理科,陕西西安710032

出　　处：《临床口腔医学杂志》2002年第6期403-405,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金 (30 0 70 81 8)

摘　　要：目的　探讨蛋白激酶A(cAMP -dependentproteinkinase ,PKA)在大鼠成骨细胞中的表达情况。方法　纯化培养大鼠成骨细胞后 ,以cAMP、PKI、rhBMP -2分别刺激细胞 3 0min ,设空白对照 ,通过免疫细胞化学及图象分析方法 ,观察细胞内PKA的活化表达情况。结果　各实验组均可见PKAα的活化表达 ,但是空白对照组表达弱(p <0 .0 5 ) ,PKI组最弱 (p <0 .0 1) ,cAMP组表达最强 (p <0 .0 1)。结论　在大鼠成骨细胞中存在功能状态的PKAα ,而且 ,rhBMP -2能激活PKAα。rhBMPObjective To observe the expression and signal significance of protein kinase A on osteoblast cells in rats .Methods After being purified ,the cultured rat osteoblast cells were treated with cAMP , PKI , rhBMP-2 for 30 minutes . The expressions of PKAα were observed through immunocytochemical methods and image analysis. Results The expressions of PKAα were showed in cAMP, PKI, BMP and control group . But PKAα was weaker in control group( p < 0.05 ),weakest in PKI group( p < 0.01 )and strongest in cAMP group( p < 0.01 ).Conclusion PKAα is in functional status on osteoblast cells in rats. rhBMP-2 can activate PKAα.There is 'cross-talk' between BMP signal pathway and PKA signal pathway .

关 键 词：大鼠 成骨细胞 蛋白激酶A 免疫细胞化学 

分 类 号：R68[医药卫生—骨科学] R336[医药卫生—外科学]