硫酸铵梯度法制备米托蒽醌长循环脂质体  

Studies on preparation of long circulating mitoxantrone liposomes with transmembrane ammonium sulfate gradients

作　　者：黄园[1] 段逸松[1] 于波涛[1] 张志荣[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《中国药学杂志》2002年第12期917-919,共3页Chinese Pharmaceutical Journal

基　　金：国家杰出青年科学基金资助项目 ( 3992 50 39)

摘　　要：目的　利用跨膜硫酸铵梯度法制备米托蒽醌长循环脂质体并对其质量进行评价。方法　采用乙醇注入合并硫酸铵梯度法制备米托蒽醌长循环脂质体。用两亲性聚乙二醇 二硬脂酰磷脂乙醇胺 (PEG DSPE)修饰脂质体膜 ,并用SephadexG50葡聚糖凝胶柱分离 ,紫外分光光度法测定米托蒽醌脂质体的包封率 ,以激光散射粒径分析仪和电子显微镜测定米托蒽醌脂质体的粒径。结果　长循环脂质体平均粒径为 60nm ,药物包封率为 93 .65 %。脂质体外观圆整而均匀。结论　采用乙醇注入合并硫酸铵梯度法可制得包封率高、粒径小的脂质体 ,而且方法简便易行。OBJECTIVE: To investigate the preparation of the long circulating mitoxantrone liposomes with ammonium sulphate gradients method and to evaluate the quality of the liposomes. METHODS: The long circulating mitoxantrone liposomes were prepared by ammonium sulphate gradients method. The amphipathic polyethylene glycol-distearoyl phosphatidylethanolamine (PEGDSPE) was added to modify the membrane of the liposomes. Ultraviolet spectrophotometry was utilized to determine the drug entrapping efficiency of liposomal mitoxantrone. Electroscopy and laser particle analyzer were applied to determine the size of mitoxantrone miposomes. RESULTS: The mean diameter of the long circulating mitoxantrone liposomes was 60 mn, with the entrapping efficiency of 93.65%. The liposomes had perfect shape. CONCLUSION: The liposomes with high entrapping efficiency and small particle size could be prepared by ammonium sulphate gradients method, which is applied easily.

关 键 词：米托蒽醌 硫酸铵梯度法 长循环脂质体 

分 类 号：R943[医药卫生—药剂学]