肿瘤坏死因子β基因多态性在中国汉族SLE病人中的分布特点及与不同人种的比较研究  

Comparative study of gene polymorphism distribution of tumor necrosis factor-beta in Chinese Han population with systemic lupus erythematosus and different race

作　　者：鞠少卿[1] 朱健华[1] 钱绩虎[2] 王惠民[1] 张冬雷[1] 

机构地区：[1]南通医学院附属医院检验科,南通226001 [2]南通医学院免疫学教研组

出　　处：《中国免疫学杂志》2002年第12期855-857,共3页Chinese Journal of Immunology

摘　　要：目的 :探讨中国江苏地区汉族人群TNFβ基因多态性在SLE病人中分布特点 ,并与不同人种进行比较研究。 方法 :收集江苏地区 16 8名无血缘关系健康个体及 6 6例SLE病人的静脉血提取DNA ,应用聚合酶链反应限制性片段长度多态性(PCR RFLP) ,分析TNFβ基因的多态性。 结果 :中国汉族SLE病人与白种SLE病人TNFβ基因频率差异无统计学意义 ;中国汉族健康个体TNFβ 1等位基因频率明显高于白种健康个体 ;SLE病人TNFβ 2基因频率较正常人明显升高 (SLE病人 6 7 4% ,正常人 5 5 1% ,P <0 0 5 ,R =1 6 8) ;中国汉族SLE病人与白种SLE病人TNFβ基因频率差异无统计学意义。 结论 :TNFβ等位基因频率在正常人分布具有种族差异 ,但在SLE病人分布无种族差异。Objective:To investigate the gene polymorphism distribution of tumor necrosis factor-beta(TNFβ) with SLE patient and different race in Chinese Han population of Jiangsu province.Methods:DNA samples were extraced from 168 EDTA-blood of unrelated healthy individuals and 66 SLE patients.TNFβ alleles were typed using polymerase chain reaction-restrication fragment length polymorphism(PCR-RFLP). Results:The alleles frequencies of TNFβ was higher significantly in Chinese Han population than in the White race;the gene frequencies of the TNFβ*2 was higher significantly in SLE patients than in the normal controls(SLE patients 67.4%,normal controls 55.1%,P<0.05,R=1.68),but the gene frequencies of the TNFβ wasn't different significantly in SLE patients between Chinese Han population and White race.Conclusion:There exist ethnic difference in the distribution of TNFβ alleles in healthy individuals;but there are no differences in SLE patients between the different race.

关 键 词：肿瘤坏死因子Β 基因多态性 聚合酶链反应 限制性片段长度 SLE 系统性红斑狼疮 

分 类 号：R593.241[医药卫生—内科学]