抗生素对猕猴桃叶柄愈伤组织诱导及分化的影响  

Effect of Antibiotics on Callus Induction and Differentiation in Actinidia chinensis

作　　者：黄萍[1] 沈孝善[1] 马朝宏[1] 

机构地区：[1]贵州省农业科学院生物技术研究所,贵阳550006

出　　处：《贵州农业科学》2002年第6期6-7,共2页Guizhou Agricultural Sciences

摘　　要：以中华猕猴桃 79- 2组培苗叶柄为实验材料 ,将其分别接在含不同浓度及类型的抗生素的培养基 (MS+ 6 μmol/L ZT+ 12 μmol/L IAA) [1]中 ,于 2 5℃、12 h光照下培养。结果 :(1)发现卡那霉素对猕猴桃叶柄愈伤组织诱导及分化的影响最敏感 (大于或等于 2 5 mg/L就无不定芽分化 ) ,四环素次之 ,链霉素影响第三 ;(2 )抑制细菌细胞壁合成的抗生素 (如青霉素、头孢霉素等 ) ,当其浓度范围分别在 0～ 5 0 0 mg/L时 ,叶柄均能在短期内形成愈伤组织并分化出芽 ,随着浓度的增加 ,差别不显著。In this Study, leaf stalks of Chinese gooseberry was used as experimental materials for tissue culture on the medium with different Antibiotics. The results showed: (1) The efficiency of Kanamycin on the callus induction and differentiation was best but Streptomycin was worst and Tetracycline Hcl is middle. (2) The antibiotics for inhibithing bacterial cell wall synthesis, such as Penicillin, Ampicillin, Carbenicillin and Cefotarime, their efficiency on the callus induction and differentiation are not different.

关 键 词：叶柄 愈伤组织 猕猴桃 抗生素 诱导 分化 遗传转化 

分 类 号：S663.4[农业科学—果树学]