单纯疱疹病毒糖蛋白单克隆抗体对致死性腹腔感染BALB/c幼鼠的被动保护作用  

Protection against Lethal Challenge of BALB/c Mice by Passively Transferred Monoclonal Antibodies to Glycopro-teins of Herpes Simplex Virus

作　　者：刘雪雁[1] 汪美先[2] 秦克锋[2] 黄艳 喻启桂[2] 郑仁瑞[2] 

机构地区：[1]总后医学专科学校微生物学教研室,北京100071 [2]第四军医大学微生物学教研室,西安710032

出　　处：《单克隆抗体通讯》1992年第1期14-18,共5页

摘　　要：用单纯疤疹病毒(HSV)Ⅰ型SM44株和Ⅱ型SaV株分别腹腔感染BALB/c小鼠,于感染前后不同时间经腹腔注射HSV单克隆抗体(McAb)观察6株McAbs对致死性腹腔感染小鼠的被动保护作用。结果4株McAbs(2C5、1A12、Mad-2、1D10)对HSV-Ⅰ感染的小鼠有保护作用,5株McAbs(2C5、1A12、2A8、1D10、CH-A9)对HSV-Ⅱ感染的小鼠有保护作用。体内保护作用与体外的中和活性相关;并分析了McAbs在中和试验中有无补体参与条件下的保护能力。还证实了HSV糖蛋白在急性感染病程中其型特异性和型共同性抗原决定簇在体内的表达。BALB/c mice were intraperitoneally challenged with the SM44 strain of HSY-1 and the say strain of HSV-2, respectively. At the time of challenge or 24 hou- rs later, 6 monoclonal antibodies directed against glycoproteins of herpes simplex virus were administered intraperitoneally for evaluation of their passive protection of the animals against lethal challenge. It was found that 4 McAbs ( 2C5, 1A12, Mad-2 and 1D10.) might protect against HSV-1 infection while 5 McAbs ( 2C5, 1A12, 2A8, 1D10 and CH-A9 ) might protect against HSV-2 infection. A direct correlation was observed between in vitro neutralization and in vivo protection, protection against HSV infection by the monoclonal antibodies was analyzed by neutralization tests with or without complement participation. The results confirmed that the glycoproteins of herpes simplex virus expressed both type-common and type-specific determinants during the course of acute virus infection in vivo.

关 键 词：单纯疱疹病毒 糖蛋白类 单克隆抗体 

分 类 号：R511.03[医药卫生—内科学]