抗人红细胞生成素单克隆抗体的研制  

Preparation of Monoclonal Antibobies against Erythropoietin (EPO)

作　　者：官奇 于爱新[1] 蔡辉国[1] 汤晓培[1] 卞钟元 贾海蓉[1] 梁强 马双[1] 沈德诚[1] 陈璋[1] 蔡英林[1] 

机构地区：[1]中国医学科学院血液学研究所,天津300020

出　　处：《单克隆抗体通讯》1992年第4期8-10,共3页

基　　金：国家七五攻关课题

摘　　要：红细胞生成素(EPO)在临床和基础研究中应用广泛。本文用从人尿中提取的EPO免疫动物制备出两株分泌抗EPO单克隆抗体的杂交瘤细胞系,分别命名为HIEPO2和HIEPO11。经鉴定HIEPO2和HIEPO11均能与重组人EPO(rHuEPO)反应,其免疫球蛋白类型均为IgM,用HIEPO2和HIEPO11制备的亲和层析柱纯化人尿中EPO,前者效果优于后者。HIEPO2对EPO的活性有抑制作用,HIEPO11对EPO的活性无影响。Erythropoietin ( EPO ) is widely used in clinical and basic studies. In the present experiment, two hybridoma cell lines (HIEPO2 and HIEPO11 ) secreting monoclonal antibody against EPO were produced by fusion of mouse myeloma cells ( NS1 ) and spleen cells from BALB/c mice immunized with EPO purified from human urine. It was found that both the McAbs were bound to recombinant human EPO arid their immunoglobulin isotype subclass was IgM.. The quantity and toxicity of EPO purified by HIEPO2 were greater than that byHIEPO11, Moreover, HIEPO2 may suppress the activity of EPO while HIEPO11l cannot.

关 键 词：红细胞生成素 单克隆抗体 

分 类 号：R392－33[医药卫生—免疫学]