闹羊花与野菊花红外光谱的分析与鉴定  

The Analyses and Identification of Flos Rhododendri Mollis and Flos Chrysanthemi Indici Via Infrared Spectroscopy

作　　者：金哲雄[1] 王玥[1,2] 周群[2] 陈建波[2] 马芳[2] 孙素琴[2] 

机构地区：[1]哈尔滨商业大学药学院中药学,黑龙江哈尔滨150001 [2]清华大学化学系,北京100084

出　　处：《光谱学与光谱分析》2014年第9期2434-2438,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(31370556);国家教育部博士点基金项目(20132332110004)资助

摘　　要：采用傅里叶变换红外光谱对杜鹃花科植物闹羊花与菊科植物野菊花两种花类中药材的主成分进行表征,其中闹羊花出现在1 648和1 543cm-1出现明显的酰胺Ⅰ带和酰胺Ⅱ带吸收峰,证明其中含有蛋白质,可能与其具有的免疫作用相关;野菊花出现在1 734和1 515cm-1等CO伸缩振动吸收峰,证明其中含有挥发油等酯类成分。由于二者的红外光谱具有较高的相似性且难以区分,通过二级鉴别比较闹羊花与野菊花的二阶导数红外光谱可看出,在1 656和1 515cm-1附近的吸收峰,表明二者均含有黄酮类成分;野菊花的二阶导数谱图中出现在1 163,1 077,1 026,986和869cm-1的吸收峰与菊糖的特征吸收峰一致,因此可以看出野菊花中含有菊糖。选取不同波数范围的闹羊花与野菊花的二维相关红外光谱进行对比,发现在1 700~1 400cm-1黄酮类成分区域闹羊花有3个明显的自动峰,野菊花有10个明显的自动峰;在1 250~900cm-1糖苷类成分区域闹羊花有10个明显的自动峰,野菊花有9个明显的自动峰。红外光谱的'三级鉴定法'为中药材的鉴别提供更快速、准确、方便的手段。In this study,major chemical components of Flos rhododendri mollis and Flos chrysanthemi indici were characterized using Fourier transform infrared spectroscopy(FTIR).For Flos rhododendri mollis,the bands at 1 648 and 1 543cm-1 were attributed to amideⅠ and amideⅡ,respectively,indicating that it contained proteins probably resulting in immunization.In case of Flos chrysanthemi indici,stretchingvibrationof CO functiongroupwasresponsibleforthebandsat1734and1515cm-1,as a result of essential oils,lipids,etc.Since FTIR spectra of Flos rhododendri mollis and Flos chrysanthemi indici are almost identical and it is difficult to discriminate them,two-step identification was investigated via secondary derivative of the FTIR spectra.The bands at 1 656 and 1 515cm-1 corresponds to flavonoides in Flos rhododendri mollis and Flos chrysanthemi indici.In the secondary derivative of the FTIR spectrum of Flos chrysanthemi indici,characteristic bands of inulin were present at 1 163,1 077,1 026,986 and 869cm-1,and therefore Flos chrysanthemi indici contained inulin as well.Tri-step identification was carried out for Flos rhododendri mollis and Flos chrysanthemi indici by means of comparing their 2D-IR correlation spectra in different wave number range.In the characteristic range of flavonoides(1 700~1 400cm-1),Flos rhododendri mollis exhibited 3obvious autopeaks,while 10 autopeaks were visualized in the 2D-IR correlation spectrum of Flos chrysanthemi indici Moreover,in the characteristic range of glucoside(1 250~900cm-1),10 and 9autopeaks were present in the 2D-IR correlation spectra of Flos rhododendri mollis and Flos chrysanthemi indici,respectively.Therefore,the tri-step identification of FTIR is a time-saving,accurate,cost-saving and convenient method to effectively distinguish traditional Chinese medicines.

关 键 词：闹羊花 野菊花 傅里叶变换红外光谱 二阶导数红外光谱 二维相关红外光谱 

分 类 号：R284.1[医药卫生—中药学] O657.33[医药卫生—中医学]