家兔UCP2基因多态性的筛选和生物信息学研究  

Study on the SNPs of Rabbit UCP2 Gene and Bioinformatics Research

作　　者：李维[1,2] 刘若余[1,2] 冯艳青 李思[1,2] 杜雪琴 谢海强 肖超能[1,2] 林家栋[1,2] 

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室 [2]贵州大学动物科学学院贵州省动物遗传育种与繁殖重点实验室,贵阳550025 [3]贵州铜仁万山区农牧科技局,铜仁554300 [4]贵阳市花溪区第三实验学校,贵阳550006

出　　处：《基因组学与应用生物学》2015年第4期700-705,共6页Genomics and Applied Biology

基　　金：贵州省科技支撑计划项目(农业攻关,黔科合NY字[2012]3013号)资助

摘　　要：本文研究兔的UCP2基因的多态性,为地方兔种的选种和选育提供一定的依据。分别以比利时兔、加利福尼亚兔和新西兰兔构建其DNA池,设计6对引物扩增3个兔种UCP2基因的外显子序列和部分内含子序列,用PCR产物直接进行双向测序快速的筛选出兔的UCP2的多态位点。结果表明:在兔UCP2基因中筛选到8个多态性位点:intron2-G2217T、exon3-A63C、exon3-G169A、intron5-C61A、exon6-C11G、intron6-C7T、intron6-C46T、intron6-C73A,其中exon3-A63C和exon3-G169A位于第3外显子上,且exon3-A63C为错义突变,导致编码的酪氨酸(Tyr)变为丝氨酸(Ser)。exon6-C11G在第6外显子上,其余的多态位点都在内含子中,除了intron6-C7T外,其余的多态位点在三种兔种中都有。结论:通过生物信息学对兔UCP2基因的分析发现m RNA二级结构、二级结构和蛋白质的三级结构在突变前后都发生变化。This study was polymorphisms in UCP2 gene which could lay the experiment foundation for heredity selection of rabbit. Respectively in Belgian Hare, California rabbit and New Zealand rabbit build their DNA pools,The complete sequences of UCP2 gene were obtained by exon designing six pair specific primers. Then by conducting bidirectional sequencing after purification of PCR production subsequently. Result: the results showed that eight SNPs(intron2-G2217 T, exon3-A63 C, exon3-G169 A, intron5-C61 A, exon6-C11 G, intron6-C7 T, intron6-C46 T, intron6-C73A) were found in UCP2 which included one missense mutation(exon3-A63 C, Tyr-Ser),Exon3 A63 C and exon3 G169 A located in exon 3, Exon6-C11 G in exon 6, the rest of the polymorphic loci in the introns, besides intron6-C7 T, the rest of the polymorphic loci in three plasticne. Conclusion: through bioinformatics to analyze the impact of secondary structure of m RNA, protein secondary structure and tertiary structure of UCP2 gene.

关 键 词：UCP2基因 比利时兔 新西兰兔 加利福尼亚兔 SNPS 

分 类 号：S829.1[农业科学—畜牧学]