异盘并殖吸虫发育过程同工酶谱分析  

Analysis on Patterns of Isozyme in Different Development Stages in Paragonimus heterotremus

作　　者：毛玮[1,2] 刘登宇[1,3] 胡文庆[1] 

机构地区：[1]广西医科大学基础医学院寄生虫学教研室,南宁530021 [2]广西疾病预防控制中心,南宁530021 [3]广西医科大学基础医学研究重点实验室,南宁530021

出　　处：《基因组学与应用生物学》2015年第4期718-722,共5页Genomics and Applied Biology

基　　金：广西自然科学基金项目(桂科自0448053);广西高等学校优秀中青年骨干教师培养工程项目共同资助

摘　　要：肺吸虫病是感染并殖吸虫属肺吸虫引起的一种食源性人畜共患寄生虫病。异盘并殖吸虫是在中国南方、东南亚和印度人群中引起肺吸虫病的主要病原体。通过同工酶谱分析了解异盘并殖吸虫在宿主体内不同发育阶段生物化学代谢变化变化规律,可为防治肺吸虫病提供科学依据。本研究采用浓度梯度聚丙烯酰胺凝胶垂直板状电泳(CG-PAGSE)分析了异盘并殖吸虫五个虫龄期同工酶谱。结果表明在不同的发育时期,同工酶谱存在一定程度的变异,呈现生长发育的多态性。Paragonimiasis is an food-borne parasitic zoonosis caused by infection with lung flukes of the genus Paragonimus. Paragonimus heterotremus is the main etiological agent of human paragonimiasis in southern China,Southeast Asia and India. To understand the metabolic patterns of Paragonimus heterotremus in the different developmental stages in the host by isozyme patterns analysis will provide the scientific foundation for prevention and treatment of lung fluke disease. In this paper, the isozyme patterns of 5 different developmental stages was analysed by concentration gradient polyacrylamide gel slab electrophoresis(CG-PAGSE). Results show the isozyme patterns with polymorphism during fluke's development.

关 键 词：异盘并殖吸虫 发育过程 同工酶谱 

分 类 号：R383.2[医药卫生—医学寄生虫学]