N-氨甲酰谷氨酸对小鼠的亚慢性毒性试验研究  

Study on Subchronic Toxicity Test of N-Carbamylglutamate on Mouse

作　　者：洪伟鸣[1] 曹飞[2] 宋亮[1] 左伟勇[1] 

机构地区：[1]江苏农牧科技职业学院江苏省兽用生物制药高技术研究重点实验室,泰州225300 [2]南京工业大学生物与制药工程学院,南京211816

出　　处：《基因组学与应用生物学》2015年第4期731-735,共5页Genomics and Applied Biology

基　　金：江苏省自然科学基金项目(BK2010557);江苏农科技职业学院2012年度院级课题(PT1201)共同资助

摘　　要：本实验研究了自行合成的N-氨甲酰谷氨酸对小白鼠的亚慢性毒性。试验组别分设高、中、低3个不同剂量组,另设空白对照组,连续90 d对小鼠灌胃1.5 mg/m L N-氨甲酰谷氨酸溶液后剖检各组存活小鼠。统计学分析结果表明,各试验组与空白对照组相比体重差异显著(p<0.05);各试验组的血常规检测指标、血液生化学检测指标的测定结果以及脏器系数与空白对照组测定结果相比差异均不显著(p>0.05);组织病理学检查显示小鼠的主要内脏器官无明显异常病变。试验结果表明,N-氨甲酰谷氨酸按推荐用量饲喂小鼠使用无毒性反应,应用安全可靠。The research is to study the subchronic toxicity of the N-carbamylglutamate on mouse. Experiment mice were divide into 4 groups, the 3 experiment groups were given 1.5 mg/m L N-carbamylglutamate liquid by oral gavage for 90 days at dose levels of 0.01, 0.02, 0.03 m L/g·body weight per day. The control group was given saline. Results showed that the body weight of experiment groups have significant difference of control group(p<0.05); The index of hematology & blood biochemistry and the organ coefficient were not significantly different from that in the control group(p>0.05). In addition, results of histopathology examination showed that no obvious abnormalities in the major organ of mice were found among experimental groups after administration of the N-carbamylglutamate. Therefore, our study suggested that the N-carbamylglutamate had no subchronic toxicity and could be safely used for experimental animals.

关 键 词：N-氨甲酰谷氨酸 小鼠 亚慢性毒性试验 

分 类 号：S859.82[农业科学—临床兽医学]