影响哺乳动物卵母细胞玻璃化冷冻保存效果的因素  

Effects of Several Factors on Cryopreservation of Mammal Oocytes by Vitrification

作　　者：王彩玲[1,2] 张华智 王利[1,2] 彭三凤 周学亮 陆阳清[1,2] 杨小淦 许惠艳[1,2] 卢晟盛 卢克焕[1,2] 

机构地区：[1]广西大学亚热带农业生物资源保护与利用国家重点实验室,南宁530004 [2]广西高校动物繁殖与动物生物技术重点实验室,南宁530004 [3]钦州市畜牧站,钦州535000

出　　处：《基因组学与应用生物学》2015年第4期849-854,共6页Genomics and Applied Biology

基　　金：国家现代农业产业技术体系广西创新团队(NYCYTXGXCXTD-04);广西高等学校高水平创新团队及卓越学者计划项目;南宁市科学研究与技术开发计划项目(20125191)共同资助

摘　　要：从屠宰场收集具有高遗传价值的卵母细胞进行冷冻保存,生产可移植胚胎,这为补充宝贵的种质资源以及物种遗传改良提供机会。虽然玻璃化冷冻技术已经成功保存了几种动物的卵母细胞,但由于卵母细胞对低温极其敏感,导致其进展十分缓慢。本文主要综述了卵母细胞脂质含量、卵丘细胞层、冷冻卵母细胞阶段、冷冻保护剂等因素对哺乳动物卵母细胞玻璃化冷冻保存效果的影响,为改善卵母细胞冷冻效果指引方向。Cryopreservation of oocytes collected from slaughtered animals of high genetic value and their subsequent utilization for production of transferable embryos provide an opportunity to replenish the valuable germplasm lost and lead to genetic improvement of the species. Although successful oocyte vitrification has been achieved in several animal species, cryopreservation of oocytes progress is still limited because of their sensitivity to the cooling and freezing process. This review summarized the effects of oocytes lipid, cumulus cells,developmental stage of oocytes and cryoprotectant on cryopreservation by vitrification, with the aim of giving directions for improve the efficiency of vitrification in oocytes.

关 键 词：卵母细胞 玻璃化冷冻 冷冻保存 效果 

分 类 号：Q954.43[生物学—动物学]