海洋链霉菌Streptomyces sp.B9173的色霉素A_3及其类似物  

Chromomycin A_3 and Its Analogues from the Marine Streptomyces sp. B9173

作　　者：徐向飞[1] 方启[1] 梁祖韬 林双君[1] 

机构地区：[1]上海交通大学生命科学技术学院微生物代谢国家重点实验室,上海200240

出　　处：《基因组学与应用生物学》2015年第5期1013-1020,共8页Genomics and Applied Biology

基　　金：教育部重大科研项目(NO.311018);国家自然基金委员会(No.31070057)共同资助

摘　　要：链霉菌(Streptomyces sp.B9173)是从智利的太平洋沿岸沉积物中分离得到的海洋放线菌,据报道它可以产生多个哌嗪二酮类生物碱化合物。在这个菌株的全基因组测序完成后,我们首先利用Neighbor-Joining法构建系统发育树,将该菌株初步鉴定为Streptomyces rishiriensis。然后,通过薄层层析、正反相硅胶柱色谱、凝胶色谱、液相半制备等技术对B9173菌株中次级代谢产物进行分离纯化,获得了3个单体化合物,并结合质谱、核磁共振技术对这3个化合物进行结构解析和鉴定。结构鉴定表明化合物1为色霉素A3,化合物2和化合物3初步确定为两个新的色霉素类似物,这为从特殊环境微生物寻找新型药物提供基础。Streptomyces sp. B9173 was isolated from the sediment collected at the pacific coast of Chile, and several diketopiperazine alkaloids were identified from this strain. After its genome was sequenced, we identified the strain B9173 to be a strain of S. rishiriensis by constructing the phylogenetic tree with the method of Neighbor-Joining. Three compounds were obtained from the fermentation broth of S. sp. B9173 by using thin-layer chromatography, normal phase and reverse phase silica flash column chromatography, gel and semi-preparative high-performance liquid chromatography. Their chemical structures were elucidated by spectroscopic technologies including nuclear magnetic resonance(NMR) and mass spectroscopy(MS). The compound 1 was identified as chromomycin A3, an antitumor agent. Compounds 2 and 3 were preliminarily determined as the analogues of chromomycin A3, and they are likely to be new compounds based on MS and proton NMR analysis. These results set stage for discovery of new compounds from the special eco-environmental microorganisms for drug screening.

关 键 词：海洋链霉菌B9173 菌种鉴定 天然产物 色霉素 色霉素类似物 

分 类 号：Q936[生物学—微生物学]