基于TALE的生物工程技术在植物抗病性上的应用  

Bio-engineering Technology Applied in Plant Disease Resistance based on TAL Effectors

作　　者：蒋国凤[1,2] 

机构地区：[1]广西大学林学院,南宁530004 [2]亚热带农业生物资源保护与利用国家重点实验室,南宁530004

出　　处：《基因组学与应用生物学》2015年第5期1103-1113,共11页Genomics and Applied Biology

基　　金：广西大学科研基金项目(XJZ130365);广西自然科学基金项目(2014GXNSFBA118075)共同资助

摘　　要：植物病原菌通过T3SS(typeⅢsecretion system)注射TALEs(transcription activator-like effectors)即转录激活类效应物进入寄主细胞并诱导寄主基因表达。TALEs通过重复氨基酸序列模块-对应识别DNA碱基序列并与之结合,激活基因转录。TALEs识别碱基的模块能够被预测和设计,可根据此推测其在植物中的功能和合成理想的靶DNA结合序列。利用植物与TALEs之间的抗性机制为疾病控制提供新的策略。Phytopathogen use a type Ⅲ secretion system(T3SS) to inject TALEs(transcription activator-like effe ctors) into plant cells where induce expression of host plant genes. TALEs activate transcription of target genes with a modular DNA-binding domain, which is a number of repeated amino acid residues on the basis of a unique code to particular DNA bases in a one-to-one correspondence. The modular is both predictable and programmable,which simplifies elucidation of TALEs function in planta and facilitates generation of novel synthetic DNA-binding domains for targeted genome manipulation. Natural mechanisms of resistance in plants against TALEs have given insights into new strategies for disease control.

关 键 词：TALE 植物-病原菌互作 黄单胞菌属 基因组基因工程 

分 类 号：S432.2[农业科学—植物病理学]