双酚A对三角涡虫DjSOD,DjCAT,DjGPx基因及相关酶活性的影响  

Effects of BPA on Activity and Gene Expression of Antioxidant Enzymes in Planarian Dugesia japonica

作　　者：邹杨建 吴素格[1] 李娟[1] 类艳贝 张秀芳[1] 

机构地区：[1]山东理工大学生命科学学院发育与进化生物学实验室,淄博255049

出　　处：《基因组学与应用生物学》2015年第6期1184-1188,共5页Genomics and Applied Biology

基　　金：山东省自然基金(No.ZR2014DM015和ZR2013CM011);国家级大学生创新创业训练计划项目(2014104-33036);国家自然基金(No.31172074)共同资助

摘　　要：本试验旨在探讨环境内分泌干扰物双酚A(BPA)对涡虫抗氧化酶(SOD,CAT,GPx)的活性及相关基因表达的影响。采用不同浓度的BPA处理涡虫48 h,然后测定抗氧化酶活性,并应用半定量RT-PCR技术探讨相关基因的变化。在BPA胁迫下,SOD活性呈现上升趋势、CAT活性表现出了先上升后下降的趋势,GPx活性总体上变化不大。随BPA浓度的增加,Dj SOD m RNA表达量上升,并呈现剂量-反应关系,Dj CAT及Dj GPx m RNA表达量也呈现出明显的上调现象,但变化趋势并不同于酶活性。BPA诱导涡虫的氧化应激反应,改变抗氧化酶SOD、CAT基因的转录水平。To understand the effect of Bisphenol A(BPA) on antioxidant enzymes(superoxide dismutase SOD, catalase CAT and glutathione peroxidase GSH-Px) activity and the m RNA levels for the genes encoding these antioxidant proteins, we used planarian as experimental material to study the antioxidant enzyme activities and the m RNA levels for the genes encoding these antioxidant proteins, under stress of different concentration of BPA for 48 h, the increase of SOD activities were most visible, meanwhile the CAT activities were found to be elevated first and then to be declined with the concentration of BPA exposure. No significant effects on glutathione peroxidase(GSH-Px)activities were observed excluding 0.564 mol/L BPA exposure. Real-time quantitative PCR showed that the transcripts of Dj SOD were up-regulated and showed dose-response relationship with the concentration of BPA exposure. The transcripts of Dj CAT and Dj GPx were up-regulated also. However, the m RNA induction patterns were not in accordance with the changes in levels of antioxidant enzymes observed. BPA exposure promotes an oxidative stress response and changes the transcription level of antioxidant enzyme genes in planarian Dugesia japonica.

关 键 词：双酚A 三角涡虫 抗氧化酶 基因表达 

分 类 号：Q78[生物学—分子生物学] Q55